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InVitro effect of pulsed 900MHz GSMradiation on mitochondrial membrane potential and motility of human spermatozoa

Abstract
Ejaculated, density purified, human spermatozoa were exposed to pulsed 900 MHz GSM mobile
phone radiation at two specific absorption rate levels (SAR 2.0 and 5.7 W/kg) and compared with
controls over time. Change in sperm mitochondrial membrane potential was analysed using flow
cytometry. Sperm motility was determined by computer assisted sperm analysis (CASA). There was
no effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential. This was also the
case for all kinematic parameters assessed at a SAR of 2.0 W/kg. However, over time, the two
kinematic parameters straight line velocity (VSL) and beat-cross frequency (BCF) were significantly
impaired (P<0.05) after the exposure at SAR 5.7 W/kg and no exposure by time interaction was
present. This result should not be ascribed to thermal effects, due to the cooling methods
employed in the RF chamber and temperature control within the incubator.

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1001256
Date January 2007
CreatorsFalzone, N, Huyser, NC, Fourie, F, Toivo, T, Leszczynski, D, Franken, D
PublisherBioelectromagnetics
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeText
FormatPdf
Rightsc 2007 Wiley-Liss, Inc.

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