Micro satellite instability (MSI) is a form of genomic instability that has recently been implicated in the pathogenesis of thyroid cancer. The purpose of this study is to further define the distribution of microsatellite instability in both normal and neoplastic thyroid follicular epithelium. / Using laser capture microdissection, cells from both normal and tumor tissue were individually collected. PCR amplification of the DNA was then performed using six dinucleotide and two mononucleotide microsatellite markers. / Forty benign and malignant thyroid tumors were compared with their adjacent normal thyroid follicular tissue and were analyzed for MSI. 9/14 papillary thyroid carcinomas and 10/16 of follicular thyroid carcinomas demonstrated MSI at >30--40% of loci tested. For benign follicular adenomas, 9/10 demonstrated microsatellite stability or low-frequency MSI. / Microsatellite instability appears to play a role in thyroid pathogenesis as evidenced by the high frequency of MSI in malignant thyroid neoplasms. In addition our study showed a significant difference in MSI frequency between follicular adenomas and follicular carcinomas. More importantly, the technique of laser capture microdissection allows for more accurate selection of benign, malignant and normal DNA.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.101730 |
| Date | January 2007 |
| Creators | Mitmaker, Elliot. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | © Elliot Mitmaker, 2007 |
| Relation | alephsysno: 002599032, proquestno: AAIMR32846, Theses scanned by UMI/ProQuest. |
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