Return to search

Identification and characterisation of a novel integrin beta subunit: Beta 7

The identification of a novel human integrin $/beta$ subunit, $/beta/sb7$, has been reported in this dissertation. The full-length human $/beta/sb7$ cDNA contains 2798 nucleotides encoding a single long open reading frame of 798 amino acid residues of which 708 are extracellular and 52 are cytoplasmic. The human $/beta/sb7$ sequence displays strong homology with other integrin $/beta$ subunits, being most related to $/beta/sb2$. Cell distribution studies suggest that the expression of transcripts encoding the human $/beta/sb7$ subunit is restricted to leukocytes. This study was the first to show that the cysteine-rich repeats of the human integrin $/beta/sb7$ subunit and other integrin $/beta$ subunits are related to domain III of the laminin B chains. Furthermore, the integrin $/beta$ subunit and laminin cysteine-rich repeats are each related to the epidermal growth factor (EGF)-like repeats found in a growing number of adhesion molecules and regulatory proteins. The mouse homologue of the human $/beta/sb7$ subunit has also been cloned in this study, and it shares 87% amino acid identity with the human $/beta/sb7$ sequence. The mouse $/beta/sb7$ mRNA transcripts, like that of the human $/beta/sb7$, have a predominantly leukocyte-restricted expression, but in some haematopoietic lineage cell lines, in addition to the predominant 3 kb transcript, a less abundant 2 kb transcript is also detectable. Cultured mouse skin epithelial cells contain only the 2 kb transcript. The genes encoding the human and mouse $/beta/sb7$ subunits were mapped to human chromosome 12 (ITGB7) and mouse chromosome 15 (Itgb-7). The Itgb-7 gene was localised to the distal part of mouse chromosome 15, and exists in the gene order: Myc-Wnt-1-Itgb-1. The N-terminal 13 amino acids of the mature mouse $/beta/sb7$ subunit was shown to be identical to a 13 amino acid N-terminal sequence reported for the Mr 120,000 $/beta$ subunit of the M290 antigen, which is found highly expressed on mouse intestinal intraepithelial lymphocytes (IEL). This finding suggests that the integrin $/beta/sb7$ subunit may play important roles in IEL migration, and immunosurveillance in the gut mucosa. / Subscription resource available via Digital Dissertations only.

  1. http://hdl.handle.net/2292/10
Identiferoai:union.ndltd.org:ADTP/246558
Date January 1992
CreatorsYuan, Qian
PublisherResearchSpace@Auckland
Source SetsAustraliasian Digital Theses Program
LanguageEnglish
Detected LanguageEnglish
Sourcehttp://wwwlib.umi.com/dissertations/fullcit/9229544
RightsSubscription resource available via Digital Dissertations only. Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated., http://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm, Copyright: The author

Page generated in 0.0019 seconds