The corresponding isoperoxidases from the flax genotrophs L and S have different molecular weights. Since further structural analysis will rely on knowledge of the primary structures of these molecules, the isolation of a molecular clone of this enzyme was attempted. Probing a cDNA library with a synthetic oligonucleotide was not successful, but a partial cDNA sequence was isolated by probing an S genotroph stem $ lambda$gt11 library with polyclonal antibodies raised against anionic plant peroxidases. Its longest reading frame sequence is divergent from those of other peroxidases, but contains some related segments. Genomic Southern analysis of the clone is presented, along with a study of the expression of its corresponding mRNA in plant tissues. A study on the effects of IAA, ABA and kinetin levels on the expression of peroxidase and of the putative peroxidase cloned mRNA is also presented.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59299 |
| Date | January 1989 |
| Creators | Beaulieu, Normand |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001067559, proquestno: AAIMM63513, Theses scanned by UMI/ProQuest. |
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