by Wong Mee-wa. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 100-104. / Contents / Abstract / Abbreviations / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Biological Functions and Structure of GH --- p.1 / Chapter 1.3 --- Molecular Cloning of GH cDNA and its Expression --- p.3 / Chapter 1.4 --- Molecular Cloning of the Genomic Sequence of Growth Hormone --- p.5 / Chapter 1.4.1 --- The Isolation of Mammalian GH gene --- p.5 / Chapter 1.4.2 --- Molecular Cloning of Rainbow Trout Growth Hormone Gene --- p.6 / Chapter 1.4.3 --- Molecular Cloning of Atlantic Salmon Growth Hormone Gene --- p.9 / Chapter 1.4.4 --- Molecular Evolution of GH gene --- p.9 / Chapter 1.4.5 --- Control Elements on GH gene --- p.12 / Chapter 1.4.6 --- Production of Transgenic Fish --- p.14 / Chapter 1.5 --- Purpose of present Study --- p.15 / Chapter Chapter 2 --- Construction of a Genomic Library --- p.17 / Chapter 2.1 --- Introduction --- p.17 / Chapter 2.2 --- Strategy --- p.19 / Chapter 2.3 --- Materials and Methods --- p.20 / Chapter 2.3.1 --- Materials --- p.20 / Chapter 2.3.2 --- Procedure --- p.24 / Chapter 2.3.2.1 --- Extraction of Total Genomic DMA --- p.24 / Chapter 2.3.2.2 --- Southern Blotting and Hybridization --- p.25 / Chapter 2.3.2.3 --- Extraction of the GH Enriched DNA Fraction --- p.28 / Chapter 2.3.2.4 --- Cloning into Phage Vector Lambda GT11 --- p.29 / Chapter 2.3.2.5 --- Studies on the Enriched Genomic Library --- p.31 / Chapter 2.4 --- Result --- p.33 / Chapter 2.5 --- Discussion --- p.43 / Chapter Chapter 3 --- Screening --- p.46 / Chapter 3.1 --- Strategy --- p.46 / Chapter 3.2 --- Materials and Methods --- p.47 / Chapter 3.2.1 --- Materials --- p.47 / Chapter 3.2.2 --- Procedure --- p.48 / Chapter 3.2.2.1 --- Primary Screening --- p.48 / Chapter 3.2.2.2 --- Purificaiton of Positive Signal --- p.49 / Chapter 3.2.2.3 --- DNA Extraction from Positive Clone --- p.49 / Chapter 3.3 --- Result --- p.50 / Chapter 3.4 --- Discussion --- p.58 / Chapter Chapter 4 --- Studies on the Positive Clone --- p.59 / Chapter 4.1 --- Introduction --- p.59 / Chapter 4.2 --- Materials and Methods --- p.60 / Chapter 4.2.1 --- Materials --- p.60 / Chapter 4.2.2 --- Procedure --- p.62 / Chapter 4.2.2.1 --- Insert DNA Preparation --- p.62 / Chapter 4.2.2.2 --- Single Enzyme Digestion --- p.62 / Chapter 4.2.2.3 --- Double Enzyme Digestion --- p.62 / Chapter 4.2.2.4 --- Preparation of Position Specific Probes --- p.62 / Chapter 4.2.2.5 --- Hybridization with Position Specific Probes --- p.64 / Chapter 4.2.2.6 --- Preparation of Competent Cells --- p.65 / Chapter 4.2.2.7 --- Subcloning into pUC18 --- p.65 / Chapter 4.2.2.8 --- Plasmid Preparation --- p.66 / Chapter 4.2.2.9 --- Sequencing --- p.67 / Chapter 4.3 --- Result --- p.69 / Chapter 4.4 --- Discussion --- p.78 / Chapter Chapter 5 --- General Discussion --- p.80 / Appendix A --- p.83 / Appendix B --- p.93 / References --- p.100
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318558 |
| Date | January 1990 |
| Contributors | Wong, Mee-wa., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 104 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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