Bone morphogenetic protein 4 (Bmp4) is a multi-functional, developmentally regulated gene and is essential for early mouse development. Little is known about the transcriptional regulation of Bmp4. To investigate the hypothesis that Bmp4 utilizes numerous long-range cis-regulatory elements to direct its repertoire of spatiotemporal expression patterns, we surveyed a 398 kilobase region of the Bmp4 locus for transcriptional activity. Our findings indicate multiple tissue-specific cis-regulatory elements reside greater than 28 kilobases 5' or 3' to the mouse Bmp4 transcription unit. We used comparative analyses to identify three noncoding sequences conserved across 450 million years of evolution that reside ~50-100 kilobases from the Bmp4 promoter and are maintained in a syntenic group across vertebrates. One of three ancient noncoding sequences reproducibly directed lacZ expression in embryonic mesoderm. Taken together, these experiments indicate an ancient, mesoderm-specific Bmp4 cis-regulatory element resides nearly 50 kilobases 5' to mouse Bmp4.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-07072008-144634 |
| Date | 04 August 2008 |
| Creators | Chandler, Kelly Jane |
| Contributors | Linda Sealy, Maureen Gannon, Richard O'Brien, E. Michelle Southard-Smith, Ela Knapik |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-07072008-144634/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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