Keung, Yiu Cheung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 58-59). / Abstracts in English and Chinese. / Contents --- p.i / Acknowledgement --- p.iii / Abstract --- p.iv / Abbreviations and Acronyms --- p.vi / Chapter Chapter 1 - --- Amphiphilic Dendrons and Gene Transfection / Chapter 1.1 --- Introduction to Dendrimers and Gene Transfection --- p.1 / Chapter 1.2 --- Overview of Gene Transfection Vectors in the Literature --- p.4 / Chapter 1.2.1 --- Viral Vectors --- p.5 / Chapter 1.2.2 --- Non-viral Vectors --- p.5 / Chapter 1.2.2.1 --- Cationic Lipids --- p.6 / Chapter 1.2.2.2 --- Cationic Polymers --- p.7 / Chapter 1.2.2.3 --- Cationic Dendrimers --- p.8 / Chapter Chapter 2 - --- En Route to Dendritic Amphiphilic Vectors / Chapter 2.1 --- Introduction to the Design of Dendritic Amphiphiles --- p.13 / Chapter 2.2 --- Barriers to Gene Transfection --- p.13 / Chapter 2.2.1 --- Cellular Barriers --- p.14 / Chapter 2.2.2 --- Extracellular Barriers --- p.16 / Chapter 2.2.3 --- Material Design Considerations --- p.17 / Chapter 2.3 --- Amphiphilic Vector Design Strategy --- p.18 / Chapter 2.4 --- Summary --- p.21 / Chapter Chapter 3 - --- "Design, Synthesis and Structural Characterization of New Amphiphilic Dendrons for Gene Transfection" / Chapter 3.1 --- Design of the New Amphiphilic Dendrons . --- p.23 / Chapter 3.1.1 --- Incorporation of Hydrocarbon Dendrons --- p.24 / Chapter 3.1.2 --- Synthesis Feasibility --- p.24 / Chapter 3.2 --- Synthesis of the New Amphiphilic Dendrons --- p.25 / Chapter 3.3 --- Characterization of the Intermediates and Targeted Amphiphilic Dendrons --- p.28 / Chapter 3.3.1 --- 1H NMR Spectroscopy --- p.28 / Chapter 3.3.2 --- I3C NMR Spectroscopy --- p.30 / Chapter 3.3.3 --- Mass Spectrometry --- p.33 / Chapter 3.3.4 --- Critical Micelle Concentration --- p.35 / Chapter 3.4 --- Conclusion --- p.38 / Chapter Chapter 4 - --- Determination of Transfection Efficiency and Cytotoxicity / Chapter 4.1 --- Preparation of the Amphiphilic Dendrons for Transfection --- p.39 / Chapter 4.2 --- Transfection Efficiencies of the Amphiphilic Dendrons 1-10 --- p.39 / Chapter 4.3 --- Cytotoxicity Assay --- p.42 / Chapter 4.4 --- Conclusions --- p.44 / Chapter Chapter 5 - --- Experimental Procedures / Chapter 5.1 --- General Information --- p.46 / Chapter 5.2 --- Experimental Procedures --- p.47 / References --- p.58 / Appendix / NMR Spectra --- p.60
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327436 |
| Date | January 2011 |
| Contributors | Keung, Yiu Cheung., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, vii, 91 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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