Tang Kwan-Yee. / Thesis submitted in: September 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 86-94). / Abstracts in English and Chinese. / TABLE OF CONTENTS --- p.i / LIST OF FIGURES --- p.v / LIST OF TABLES --- p.viii / ABSTRACT --- p.ix / ACKNOWLEDGEMENT --- p.xiii / DECLARATION --- p.xiv / ABBREVIATIONS --- p.xv / Chapter CHAPTER ONE --- INTRODUCTION / Chapter 1.1. --- Structure and conformation of Curdlan --- p.1 / Chapter 1.2. --- Important of Curdlan --- p.3 / Chapter 1.3. --- Conventional methods on molecular weight and molecular weight distribution on polysaccharide --- p.4 / Chapter 1.3.1. --- Laser Light Scattering --- p.4 / Chapter 1.3.2. --- Size Exclusion Chromatography --- p.5 / Chapter 1.3.3. --- Viscometry --- p.6 / Chapter 1.4. --- Polysaccharide analysis using MALDI-TOF-MS --- p.7 / Chapter 1.5. --- Matrix-Assisted Laser Desorption／Ionization Mass Spectrometry --- p.9 / Chapter 1.6. --- Outline of project --- p.13 / Chapter CHAPTER TWO --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1. --- Instrumentation --- p.14 / Chapter 2.1.1. --- Vacuum system --- p.14 / Chapter 2.1.2. --- Laser-based Ion Source --- p.14 / Chapter 2.1.3. --- Time-of-flight Mass Analyzer --- p.18 / Chapter 2.1.4. --- Detector and Data System --- p.22 / Chapter 2.2. --- Experimental --- p.22 / Chapter 2.2.1. --- Extraction of water-soluble and water-insoluble Curdlan --- p.22 / Chapter 2.2.2. --- Fractionation of water-soluble Curdlan samples --- p.23 / Chapter 2.2.3. --- Fractionation of water-insoluble Curdlan samples --- p.23 / Chapter 2.2.4. --- Phenol-sulfuric acid test --- p.24 / Chapter 2.2.5. --- Sample preparation in MS --- p.24 / Chapter 2.2.6. --- Calibration of MALDI-TOFMS --- p.24 / Chapter 2.3. --- Data Analysis --- p.25 / Chapter CHAPTER THREE --- OPTIMIZATION OF MALDI EXPERIMENTAL CONDITIONS FOR ANALYSIS OF CURDLAN / Chapter 3.1. --- Introduction --- p.27 / Chapter 3.2. --- Experimental --- p.28 / Chapter 3.2.1. --- Sample preparation method for analysis of water-soluble Curdlan --- p.29 / Chapter 3.2.2. --- Sample preparation method for analysis of water- insoluble Curdlan --- p.29 / Chapter 3.2.2.1 --- Aqueous DHB matrix --- p.30 / Chapter 3.2.2.2 --- Aqueous DHB/NH4F matrix --- p.30 / Chapter 3.2.2.3 --- Non-aqueous DHB matrix (in DMSO) --- p.30 / Chapter 3.2.2.4 --- Non-aqueous DHB/3AQ matrix (in DMSO) --- p.30 / Chapter 3.2.2.5 --- Aqueous DHB/3AQ matrix --- p.31 / Chapter 3.2.3. --- Fractionation of the water-soluble and water-insoluble Curdlan --- p.31 / Chapter 3.3. --- Results and Discussion --- p.32 / Chapter 3.3.1. --- Development of matrix system for analysis of water- insoluble Curdlan --- p.35 / Chapter 3.3.2. --- MALDI analysis of water-soluble and water-insoluble Curdlans --- p.39 / Chapter 3.3.3 --- MALDI analysis of fractionated water-soluble and water- insoluble Curdlans --- p.42 / Chapter 3.4. --- Conclusion --- p.48 / Chapter CHAPTER FOUR --- "EVALUATION OF MW AND MWD OF DMSO- SOLUBLE CURDLAN BY MALDI-TOF-MS, UV- VIS, REFRACTOMETRIC AND GC-MS ANALYSIS" / Chapter 4.1. --- Introduction --- p.51 / Chapter 4.2. --- Experimental --- p.54 / Chapter 4.2.1. --- Gel permeation chromatography of water-insoluble Curdlan --- p.54 / Chapter 4.2.2. --- Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry --- p.55 / Chapter 4.2.3. --- Ultraviolet Absorption Spectrometry (UV-VIS) --- p.55 / Chapter 4.2.4 --- Refractometry Analysis (RI) --- p.56 / Chapter 4.2.5 --- Gas Chromatography Mass Spectrometry (GC-MS) --- p.56 / Chapter 4.3. --- Results --- p.57 / Chapter 4.3.1 --- MALDI analysis --- p.57 / Chapter 4.3.2 --- Ultra-violet Absorption Spectrometry (UV-VIS) --- p.61 / Chapter 4.3.3 --- Refractometry Analysis (RI) --- p.62 / Chapter 4.3.4 --- Gas chromatography Mass Spectrometry (GC-MS) --- p.66 / Chapter 4.4. --- Discussion --- p.79 / Chapter 4.5 --- Conclusion --- p.83 / Chapter CHAPTER FIVE --- CONCLUDING REMARKS --- p.84 / REFERENCES --- p.86 / APPENDIX / Appendix A Chemical Structure of Curdlan --- p.95 / Appendix B Structure of Universal Matrices Used in MALDI-TOF- MS --- p.96 / "Appendix C Structure of Aniline Blue fluorochrome Sodium 4,4- [carbonyldis-(benzene-4,l-diyI)bis(imino)]bisbenzene- sulphonate" --- p.97 / Appendix D Structure of common internal standard for GCMS analysis --- p.97 / Appendix E Structure of Trim ethylsilylated reagent and Trimethylsilylated glucose --- p.98 / Appendix F The table of raw data for graph plotting in RI analysis. --- p.99 / Appendix G The table of raw data used for graph plotting in UV-VIS analysis. --- p.100 / Appendix H The table of raw data used for graph plotting in GC-MS analysis. --- p.101
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324465 |
| Date | January 2003 |
| Contributors | Tang, Kwan-yee., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 94, [7] leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
Page generated in 0.0022 seconds