Wong Hei Yi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 157-175). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abstract 摘要 --- p.iv / List of Figures --- p.vi / List of Tables --- p.ix / List of Abbreviations --- p.x / Table of Contents --- p.xiii / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Importance of Glucose in Biological System --- p.1 / Chapter 1.2 --- Glucose Transporter Families --- p.2 / Chapter 1.2.1 --- Na+-Dependent Glucose Transporters --- p.2 / Chapter 1.2.2 --- Facilitative Glucose Transporters --- p.3 / Chapter 1.3 --- Glucose Transporter Type1 --- p.7 / Chapter 1.3.1 --- Primary Structure --- p.7 / Chapter 1.3.2 --- Secondary Structure --- p.8 / Chapter 1.3.3 --- Membrane Topology --- p.8 / Chapter 1.3.4 --- Tertiary Structure --- p.9 / Chapter 1.3.5 --- Kinetics Properties --- p.11 / Chapter 1.3.6 --- Affinity Reagents --- p.12 / Chapter 1.3.7 --- Tissue Distribution --- p.13 / Chapter 1.3.8 --- Multifunctional Property --- p.14 / Chapter 1.3.9 --- Characterization of GLUT1 Gene --- p.14 / Chapter 1.3.10 --- Regulation of GLUT1 Expression --- p.15 / Chapter 1.4 --- Glucose Transporter Type 1 and the Brain --- p.17 / Chapter 1.5 --- Glucose Transporter Type 1 Deficiency Syndrome --- p.20 / Chapter 1.5.1 --- Background of GlutlDS --- p.20 / Chapter 1.5.2 --- Clinical Features of GlutlDS --- p.23 / Chapter 1.5.3 --- Genotype-Phenotype Correlations --- p.24 / Chapter 1.5.4 --- Diagnosis --- p.26 / Chapter 1.5.4.1 --- Erythrocyte Glucose Transporter Activity --- p.26 / Chapter 1.5.4.2 --- Molecular Genetic Testing of GLUT1 Gene --- p.27 / Chapter 1.5.4.3 --- Glucose Concentration --- p.27 / Chapter 1.5.5 --- Management --- p.28 / Chapter 1.5.5.1 --- Ketogenic Diet --- p.28 / Chapter 1.5.5.2 --- Medication --- p.29 / Chapter 1.5.5.2.1 --- Glutl Activator --- p.29 / Chapter 1.5.5.2.2 --- Glutl Inhibitor --- p.29 / Chapter 1.6 --- Hypothesis and Objectives --- p.31 / Chapter Chapter 2: --- Identification of the First Two Asian GlutlDS Cases --- p.33 / Chapter 2.1 --- Materials --- p.34 / Chapter 2.1.1 --- Clinical History of Suspected GlutlDS Patients --- p.34 / Chapter 2.1.2 --- Blood Samples --- p.35 / Chapter 2.1.3 --- Reagents for Zero-trans Influx of 3-OMG Uptake in Erythrocytes --- p.35 / Chapter 2.1.4 --- Reagents for Zero-trans Efflux of 3-OMG Uptake in Erythrocytes --- p.37 / Chapter 2.1.5 --- Reagents for Glutl Gene Analysis --- p.37 / Chapter 2.1.6 --- Reagents and Buffers for Reverse Transcription --- p.38 / Chapter 2.1.7 --- Reagents and Buffers for Agarose Gel Electrophoresis --- p.39 / Chapter 2.1.8 --- Reagents for Erythrocytes Membrane Preparation and Detection --- p.41 / Chapter 2.2 --- Methods --- p.46 / Chapter 2.2.1 --- Zero-trans Influx of 3-OMG Uptake in Erythrocytes --- p.46 / Chapter 2.2.2 --- Zero-trans Efflux of 3-OMG out of Erythrocytes --- p.47 / Chapter 2.2.3 --- Glutl Protein Expression --- p.48 / Chapter 2.2.4 --- GLUT1 Gene Analyses --- p.51 / Chapter 2.2.5 --- Statistics --- p.58 / Chapter 2.3 --- Results --- p.59 / Chapter 2.4 --- Discussions and Conclusions --- p.69 / Chapter Chapter 3: --- Pathogenicity of GLUT1 Mutations --- p.78 / Chapter 3.1 --- Materials --- p.79 / Chapter 3.1.1 --- Construction of Glutl-Encoding Vectors --- p.79 / Chapter 3.1.2 --- Cell Lines --- p.80 / Chapter 3.1.3 --- "Cell Culture Media, Buffers and Other Reagents" --- p.81 / Chapter 3.1.4 --- Cell Culture Wares --- p.83 / Chapter 3.1.5 --- Reagents for Transfection --- p.83 / Chapter 3.1.6 --- Reagents for Protein Determination and Western Blot Analysis --- p.83 / Chapter 3.1.7 --- Reagents and Buffers for Flow Cytometry --- p.84 / Chapter 3.1.8 --- Reagents for 2-DOG Uptake in CHO-K1 Cells --- p.84 / Chapter 3.1.9 --- Reagents and Consumables for Confocal Microscopy --- p.85 / Chapter 3.2 --- Methods --- p.86 / Chapter 3.2.1 --- Cell Culture Methodology --- p.86 / Chapter 3.2.2 --- Construction of Glutl-Encoding Vectors --- p.87 / Chapter 3.2.3 --- Construction of Glutl Mutants --- p.91 / Chapter 3.2.4 --- Establishment of Wild Type and Mutant Glutl Expressing Cell Lines --- p.92 / Chapter 3.2.5 --- Glucose Influx Assays in CHO-K1 Cells --- p.96 / Chapter 3.2.6 --- Confocal Microscopy Studies on Glutl Cellular Localization --- p.97 / Chapter 3.2.7 --- Statistics --- p.98 / Chapter 3.3 --- Results --- p.99 / Chapter 3.4 --- Discussions and Conclusions --- p.112 / Chapter Chapter 4: --- Effects of Anticonvulsive Compounds on Cellular Glucose Transport --- p.117 / Chapter 4.1 --- Materials --- p.118 / Chapter 4.1.1 --- Cell Lines --- p.118 / Chapter 4.1.2 --- Cell Culture Media --- p.118 / Chapter 4.1.3 --- Blood Sample --- p.119 / Chapter 4.1.4 --- Anticonvulsive Compounds --- p.119 / Chapter 4.1.5 --- Reagents for Zero-trans Influx of 3-OMG Uptake in Fibroblasts --- p.120 / Chapter 4.1.6 --- Reagents for Zero-trans Influx of 2-DOG Uptake in Primary Astrocytes --- p.120 / Chapter 4.1.7 --- Reagents for Total RNA Isolation --- p.121 / Chapter 4.1.8 --- Reagents and Consumables for Real-Time PCR --- p.122 / Chapter 4.2 --- Methods --- p.123 / Chapter 4.2.1 --- Cell Culture --- p.123 / Chapter 4.2.2 --- Drug Concentrations --- p.123 / Chapter 4.2.3 --- Zero-trans Influx of 3-OMG Uptake in Erythrocytes --- p.123 / Chapter 4.2.4 --- Zero-trans Influx of 3-OMG Uptake in Fibroblasts --- p.124 / Chapter 4.2.5 --- Zero-trans Influx of 2-DOG Uptake in Primary Astrocytes --- p.125 / Chapter 4.2.6 --- Gene Expression Study --- p.127 / Chapter 4.2.7 --- Statistics --- p.130 / Chapter 4.3 --- Results --- p.131 / Chapter 4.4 --- Discussions and Conclusions --- p.148 / Chapter Chapter 5: --- General Conclusions and Future Perspectives --- p.154 / References --- p.157
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324602 |
| Date | January 2004 |
| Contributors | Wong, Hei Yi., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvi, 175 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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