This study evaluated the stability of oral bacteria in healthy subjects and documented
community shifts in smokers and oral/periodontal disease by employing PCR-RFLP,
DGGE and sequence analysis of the 16S rDNA gene from metagenomes and plate-wash
(cultured) bacteria of oral wash from 15 participants,. A stable core of bacterial DNA
fingerprint was detected within and between subjects and did not change over time when
analyzed in smokers and healthy non-smokers. Signature bands in smokers, non-smokers
and periodontal disease subjects were evident suggesting the presence of potential
indicators of health and poor oral health. Taxon diversity was higher in smokers
including members of the genera Rothia, Synechococcus, Neisseria, Thiomargarita and
Pyrobaculum but highest in periodontal disease. The two techniques successfully aligned
the subjects within appropriate categories (based on their oral microbial genetic
patterns)confirming their diagnostic suitability. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection
| Identifer | oai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_13654 |
| Contributors | Chakraborty, Shreyasee (author), Esiobu, Nwadiuto (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Science, Department of Biological Sciences |
| Publisher | Florida Atlantic University |
| Source Sets | Florida Atlantic University |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation, Text |
| Format | 105 p., application/pdf |
| Rights | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/ |
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