Thesis (MScMedSc (Pharmacology))--University of Stellenbosch, 2005. / In this thesis, research results are reported on the role of natural and synthetic
surfactants on the in vitro permeability characteristics of various chemical
compounds across porcine (buccal, bronchial, arterial, venous and rectal) and
human (vaginal) tissues. The permeability flux values of the different
compounds (arecoline, 17β-estradiol, hydrocortisone, dexamethasone,
vasopressin, oxytocin, zidovudine and isoniazid) were determined using a
continuous flow-through diffusion system. Mean steady state flux values were
compared statistically by means of a t-test at a significance level of 5% as well
as an F-test using whole curve comparisons. The results indicated that the
synthetic pulmonary surfactant Biopolsurf is an effective enhancer for the
permeation of chemical compounds through most of the tissues tested and
that molecular weight, electrostatic charge, partitioning of the molecules in
surfactant and surfactant concentration play an important role in trans
membrane diffusion.
In addition the epithelial permeability of the different types of tissues for
various chemical compounds (arecoline, 17β-estradiol, hydrocortisone,
dexamethasone, vasopressin and oxytocin) across the above tissues were
compared. The results obtained showed that the permeability flux values of
the compounds across porcine bronchial and human vaginal tissues were
consistently similar and that porcine buccal tissue had the lowest permeability
of all tissues tested. This was in agreement with previous in vitro studies. It
was concluded that a wide variation in the permeability characteristics of
different epithelia exists and that the pulmonary epithelium, due to its high
permeability, is probably the most effective epithelium for drug delivery
purposes, especially for drugs that undergo extensive gastrointestinal or
hepatic first-pass metabolism.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/1852 |
| Date | 12 1900 |
| Creators | Viljoen, Ianda |
| Contributors | Van der Bijl, P., University of Stellenbosch. Faculty of Health Sciences. Dept. of Medicine. Pharmacology. |
| Publisher | Stellenbosch : University of Stellenbosch |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | University of Stellenbosch |
Page generated in 0.0112 seconds