The multiplex PCR experiment is to amplify multiple regions of a DNA sequence at the same time by using different primer pairs. Although, in recent years, there are lots of methods for PCR primer design, only a few of them focus on the multiplex PCR primer design. The multiplex PCR primer design is a tedious task since there are too many constraints to be satisfied. A new method for multiplex PCR primer design strategy using genetic algorithm is proposed. The proposed algorithm is able to find a set of suitable primer pairs more efficient and uses a MAP model to speed up the examination of the specificity constraint. The dry-dock experiment shows that the proposed algorithm finds several sets of primer pairs for multiplex PCR that not only obey the design properties, but also have specificity.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0823104-175630 |
| Date | 23 August 2004 |
| Creators | Liang, Hong-Long |
| Contributors | Chungnan Lee, Kuo-sheng Chien, Kun-Mao Chao, Yow-Ling Shiue, Yung-Nien Sun |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823104-175630 |
| Rights | withheld, Copyright information available at source archive |
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