The effects of dibutyryl 3'; 5' cyclic monophosphate (dbcAMP) on several parameters of transformation were studied using a Kirsten sarcoma virus (Ki-MSV)-transformed mouse cell line (K-A31). Treated cells showed changes in morphology, decreased motility, saturation density and growth rate, and lost the capacity for anchorage-independent growth. In contrast to many other transformed cell lines, fibronectin and an elaborate cytoskeleton were present in K-A31 cells. The transcription of the proviral genome was examined using both reverse-transcribed and nick-translated ('3)H-DNA probes, and certain viral-specific RNAs were found restricted to the nucleus of dbcAMP-treated cells. Additive hybridization experiments suggested these RNAs were transcribed from rat-derived sequences located in the 5'-half of the proviral genome. These results are discussed with respect to the properties most closely associated with cellular malignancy, and the possible mechanism of dbcAMP-mediated reverse-transformation in K-A31 cells.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.77149 |
| Date | January 1982 |
| Creators | Ridgway, Anthony Allan Grinyer. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biology) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000139595, proquestno: AAINK58095, Theses scanned by UMI/ProQuest. |
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