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Previous issue date: 2017 / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / Introdu??o: A Distrofia Muscular de Duchenne (DMD) ? marcada pela falta da distrofina, e sua aus?ncia leva a altera??es mec?nicas da fibra muscular, resultando num processo de necrose muscular e altera??es histol?gicas. O treinamento f?sico de baixa intensidade vem sendo empregado como forma de retardar a progress?o da DMD, entretanto, ainda n?o se sabe os par?metros ben?ficos ao m?sculo distr?fico. Assim, objetivou-se elucidar os efeitos do exerc?cio terap?utico de baixa intensidade em esteira no m?sculo esquel?tico distr?fico do modelo mdx nos par?metros morfol?gicos, na morfometria dos marcadores de les?o muscular, da fibrose musclar e na constitui??o da matriz extracelular pelos col?genos tipo I e III. Metodologia: Foram estudados tr?s grupos: camundongos mdx exerc?cio (mdxE), mdx sedent?rio (mdxC) e controle saud?veis (Cc) (n=8/grupo). O grupo mdxE foi estimulado a correr em esteira horizontal motorizada para ratos, em baixa intensidade, 9m/min por 30 minutos/dia, 3 vezes/semana, por 8 semanas. Ap?s o protocolo de exerc?cio foi realizada a eutan?sia dos animais e coletado o m?sculo gastrocn?mio. Foi realizada a colora??o Hematoxilina-Eosina para an?lise dos marcadores de les?o muscular: N?cleo Central e Di?metro M?nimo de Feret; a rea??o de Picrossirius red para an?lise das fibras col?genas na fibrose muscular e a imuno-localiza??o das fibras col?genas tipo I e III. Resultados: Foram observadas altera??es histol?gicas distr?ficas caracter?sticas nos grupos mdxE e mdxC e feixes de fibras col?genas espessas no grupo mdxC. A imuno-histoqu?mica revelou presen?a de col?geno do tipo I principalmente no grupo mdxC. N?o houve diferen?a significativa entre os grupos mdxE e mdxC para fibras com n?cleo central e coeficiente de varia??o do Di?metro m?nimo de Feret. O grupo mdxE n?o apresentou diferen?a significativa em rela??o ao Cc para a porcentagem da ?rea de fibras col?genas na fibrose muscular. Conclus?es: O treino de baixa intensidade reduz a deposi??o de fibras col?genas na fibrose muscular, com fibras delgadas do col?geno tipo I e n?o altera os marcadores de les?o muscular. / Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Reabilita??o e Desempenho Funcional, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / Introduction: Duchenne muscular dystrophy (DMD) is marked by lack of dystrophin, and its absence leads to muscle fiber rupture, resulting in muscular necrosis and histological changes. Physical training of low intensity has been used to delay the progression of DMD, however, parameters of the dystrophic muscle are not yet known. The objective of this study was to elucidate the effects of a treadmill low intensity exercise on morphology, morphometric parameters of the muscle injury markers and the composition of the extracellular matrix by type I and III collagens of the dystrophic skeletal muscle of the mdx model. Methods: Three groups were studied: exercised mdx (mdxE), sedentary mdx (mdxC) and healthy controls (Cc) (n =8/group). The mdxE group was stimulated to run on a motorized horizontal treadmill for rats, with a low intensity (9 m/min for 30 minutes/day, 3 times/week) for 8 weeks. After the exercise protocol, the animals were euthanized and the gastrocnemius muscle was collected. Hematoxylin-Eosin staining was performed for analysis of muscle injury markers, Central Nucleus and Minimum Diameter of Feret, Picrossirius red reaction for analysis of collagen fibers in muscle fibrosis and immuno-localization of type I and III collagen fibers. Results: Dystrophic histopathological changes were observed in the mdxE and mdxC groups and thicker collagen fiber bundles in the mdxC group. Immunohistochemistry revealed the presence of type I collagen mainly in the mdxC group. There was no significant difference between the mdxE and mdxC groups for fibers with centrally located nuclei and coefficient of variation of the Minimum Feret Diameter. The mdxE group showed no significant difference in relation to Cc for the percentage of the area of collagen fibers in muscle fibrosis. Conclusions: The low intensity training reduced the deposition of collagen fibers in muscle fibrosis with thin fibers of type I collagen and did not alter the markers of muscle injury.
| Identifer | oai:union.ndltd.org:IBICT/oai:acervo.ufvjm.edu.br/jspui:1/1608 |
| Date | 09 August 2017 |
| Creators | Pinto, Priscilla Avelino Ferreira |
| Contributors | Machado, Thais Peixoto Gaiad, Ambr?sio, Carlos Eduardo, Magalh?es, Fl?vio de Castro, Machado, Alex Sander Dias, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Machado, Thais Peixoto Gaiad |
| Publisher | UFVJM |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFVJM, instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri, instacron:UFVJM |
| Rights | A concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto integral da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data., info:eu-repo/semantics/openAccess |
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