Mycobacter avium complex serovars 4 and 20 were cultured in the presence of [3H] fucose, [3H]-methionine, and [3H]-mannose to specifically radiolabel the oligosaccharide of the glycopeptidolipid (GPL) antigens. Distribution of radioactivity in lipid was determined by thin-layer chromatographic methods. Examination of acid hydrolysates from radiolabeled antigens revealed that [3H]-methionine incorporated into methylated sugars in polar and apolar GPL components, whereas [3H]-mannose incorporated exclusively into the oligosaccharide of polar GPL antigens. Least incorporation of radiolabel into antigens was observed with [3H]-fucose. Use of radiolabeled serovar 4 antigens in macrophage uptake studies revealed maximum uptake to be slightly above 250 gg/ 3.2 x 105 cells. Timed experiments demonstrated that GPL antigens were relatively inert to degradation by resident peritoneal macrophages.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500936 |
| Date | 08 1900 |
| Creators | Woodbury, Julie L. (Julie Lynn) |
| Contributors | Barrow, William W., Cox, David, Kester, Andrew S. |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | v, 70 leaves : ill., Text |
| Rights | Public, Woodbury, Julie L. (Julie Lynn), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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