Previous investigations have indicated that shoulder stiffness was related with the degree of inflammation and the number of myofibroblasts in subacromial bursa tissue of rotator cuff lesions. But the interaction mechanism between inflammatory cytokines and myofibroblasts induced shoulder stiffness has not been fully explored. The purpose of this research is to study the effect of IL-1 £] on myofibroblasts in rotator cuff lesions with shoulder stiffness. Myofibroblasts from subacromial bursa tissue of rotator cuff lesions were cultured with or without IL-1 £]. The gene and protein expression of £\-smooth muscle actin (£\-SMA) and prolyl 4-hydroxylase (PH-4) were determined. The results showed that high levels of PH-4 and low levels of £\-SMA were expressed in myofibroblasts cultured with IL-1 £]. PH-4 gene had higher expression in tissue of stiff patients than non-stiff patients, but £\-SMA gene expression had no significant difference. According to the results, myofibroblasts cultured with extra IL-1 £] showed increased PH-4 expression and decreased £\-SMA expression. This indicates myofibroblasts might transdifferentiate into fibroblasts via IL-1 £] effect.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0201107-102841 |
| Date | 01 February 2007 |
| Creators | Tseng, Hsin-Ling |
| Contributors | Chung-Lung Cho, Jih-Yang Ko, Ching-Mei Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0201107-102841 |
| Rights | not_available, Copyright information available at source archive |
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