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Molecular characterization of seminal plasma from boars with divergent sperm quality

Seminal plasma (SP) is the microenvironment of spermatozoa whose composition reflects semen quality and constitutes an excellent source for detecting non-invasive biomarkers. This study characterizes good vs. poor quality semen using seminal extracellular vesicles coupled proteins and miRNA. Fresh boar semen samples were screened, centrifuged, and SP supernatants were collected for near-infrared spectroscopy (NIRS) analysis. EVs were extracted from SP (SP-EVs), characterized, and those of extremely poor or good sperm quality groups were subjected to proteomics and small RNA sequencing. Significant differences were set for P

Identiferoai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-6825
Date12 May 2023
CreatorsDlamini, Notsile Hleliwe
PublisherScholars Junction
Source SetsMississippi State University
Detected LanguageEnglish
Typetext
Formatapplication/pdf
SourceTheses and Dissertations

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