Several systems were examined for the production and delivery of recombinant
vaccines for fish. C. crescentus was employed to produce a fragment of the IHNV
glycoprotein. When administered by injection to 0.5 gram rainbow trout (Oncorhynchus
mykiss), one of the fusion proteins (184 amino acids of the IHNV glycoprotein fused to
242 amino acids of the C-terminus of the Caulobacter crescentus) protected the fish
against lethal challenge with IHNV. Attenuated strains of Yersinia ruckeri were
generated using allelic exchange mutagenesis. These strains were characterized in terms
of in vitro growth characteristics and invasiveness. Attenuated E. coli and Y. ruckeri
were exploited to deliver plasmid DNA to fish cells in vitro; attenuated Y. ruckeri
bacteria were examined in vivo as bivalent vaccine delivery vehicles, either through the
expression of a fragment of the IHNV glycoprotein or by carrying a plasmid DNA
vaccine encoding the complete IHNV glycoprotein. A cell wall deficient strain
(11.29��dap) protected rainbow trout against lethal challenge with pathogenic Y. ruckeri.
Gene transfer to fish was not detected by luciferase reporter gene assays. No clear
protection from IHNV challenge was observed. / Graduation date: 2002
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/32690 |
| Date | 09 October 2001 |
| Creators | Simon, Benjamin E. |
| Contributors | Leong, Jo-Ann C. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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