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Molecular genetic studies of oligodendroglial and ependymal tumors.

by Tong Yuen Kwan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 124-141). / Abstract also in Chinese. / acknowledgements --- p.i / Abstract (English/Chinese) --- p.ii / contents --- p.vi / list of tables --- p.viii / ost of figures --- p.x / Chapter I. --- introduction --- p.1 / Chapter I.1. --- Tumors of the Central Nervous System --- p.1 / Chapter I.2. --- Histopathological Classification of Human Glial Tumors --- p.3 / Chapter I.2.1. --- Histopathology of Astrocytic Gliomas --- p.3 / Chapter I.2.1.1. --- Diffuse Astrocytomas --- p.3 / Chapter I.2.1.2. --- Others --- p.6 / Chapter I.2.2. --- Histopathology of Non-Astrocytic Gliomas --- p.6 / Chapter I.2.2.1. --- Oligodendroglial Tumors --- p.6 / Chapter I.2.2.2. --- Ependymal Tumors --- p.9 / Chapter I.3. --- Tumor Suppressor Genes --- p.14 / Chapter I.3.1. --- p53 --- p.14 / Chapter I.3.1.1. --- Historical Perspectives --- p.14 / Chapter I.3.1.2. --- Structure of p53 Gene and Protein --- p.15 / Chapter I.3.1.3. --- Functions of Wild-Type p53 Protein --- p.18 / Chapter I.3.1.4. --- Regulation and Modulation of the Functions of p53 --- p.21 / Chapter I.3.1.5. --- Mechnism of p53 Inactivation --- p.23 / Chapter I.3.1.6. --- p53 Mutation Profiles in Human Tumors --- p.25 / Chapter I.3.2. --- Novel Genes --- p.28 / Chapter I.3.2.1. --- PTEN/MMAC1 --- p.28 / Chapter I.3.2.2. --- DMBT1 --- p.31 / Chapter I.4. --- Cytogenetic and Molecular Genetic Studies in Gliomas --- p.34 / Chapter I.4.1. --- Astrocytic Gliomas --- p.34 / Chapter I.4.2. --- Non-Astrocytic Gliomas --- p.39 / Chapter I.4.2.1. --- Oligodendroglial Tumors --- p.39 / Chapter I.4.2.2. --- Ependymal Tumors --- p.46 / Chapter II. --- objectives of study --- p.49 / Chapter III. --- materials and methods --- p.52 / Chapter III.l. --- Patients and Materials --- p.52 / Chapter III.2. --- Collection of Samples --- p.57 / Chapter III.3. --- DNA Extraction --- p.58 / Chapter III.3.1. --- Extraction of Genomic DNA from Formalin-Fixed Paraffin Embedded Tissues --- p.58 / Chapter III.3.2. --- Extraction of Genomic DNA from Blood --- p.60 / Chapter III.4. --- Loss of Heterozygosity (LOH) Analysis on Chromosome 10q --- p.61 / Chapter III.4.1. --- Microsatellite Markers --- p.62 / Chapter III.4.2. --- Amplification of Target Sequences by PCR --- p.63 / Chapter III.4.3. --- Denaturing Polyaerylamide Gel Electrophoresis --- p.64 / Chapter III.4.4. --- Detection of Loss of Heterozygosity (LOH) --- p.64 / Chapter III.5. --- Mutational Analysis of p53 and PTEN/MMAC1 --- p.66 / Chapter III.5.1. --- Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) Analysis --- p.66 / Chapter III.5.1.1. --- PCR Primers --- p.66 / Chapter III.5.1.2. --- PCR Amplification of Target Sequences --- p.68 / Chapter III.5.1.3. --- Non-denaturing Polyacrylamide Gel Electrophoresis --- p.71 / Chapter III.5.2. --- Direct DNA Sequencing Analysis --- p.72 / Chapter III.5.2.1. --- Cycle Sequencing --- p.72 / Chapter III.5.2.2. --- Denaturing Gel Electrophoresis --- p.73 / Chapter III.6. --- Differential PCR for Detection of MDM2 Amplification --- p.74 / Chapter III.6.1. --- DNA Amplification by PCR --- p.74 / Chapter III.6.2. --- Polyacrylamide Gel Electrophoresis --- p.75 / Chapter III.6.3. --- Detection of Gene Amplification --- p.75 / Chapter IV. --- Results --- p.77 / Chapter IV.1. --- LOH Analysis of Chromosome l0q --- p.77 / Chapter IV.2. --- Mutational Analysis ofp53 and PTEN/MMAC1 --- p.92 / Chapter IV.3. --- Differential PCR Analysis of MDM2 Amplification --- p.103 / Chapter V. --- discussion --- p.109 / Chapter V.l. --- p53 Gene Inactivation Studies --- p.110 / Chapter V.2. --- Molecular Genetic Studies on Chromosome l0q --- p.113 / Chapter V.3. --- Microsatellite Instability in Non-Astrocytic Gliomas --- p.117 / Chapter V.4. --- Significance of This Study --- p.118 / Chapter V.5. --- Limitations of This Study --- p.119 / Chapter V.6. --- Future Studies --- p.122 / Chapter VI. --- REFERENCES --- p.124

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322524
Date January 1998
ContributorsTong, Yuen Kwan., Chinese University of Hong Kong Graduate School. Division of Anatomical and Cellular Pathology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 141 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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