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Novel Formulations of Antioxidant and Anti-inflammatory Drugs to Ameliorate Ischemic Damage Measured In Vitro

The two of major pathways that cause ischemic damage are oxidative stress and inflammation. To decreasing oxidative stress and inflammation, new anti-oxidant and anti-inflammatory agents are tested in ischemic models. In order to study ALRX828C anti-inflammatory properties, an in vivo six-day old air pouch model of inflammation was used to evaluate the anti-inflammatory potential of ALRX828C. Also, the dose response of ALRX828C for TNFα (IC50 = 30 μM) and IL-17 (IC50 = 1.3 μM) were determined by using human peripheral blood mononuclear cell cultures stimulated with ionomycin and PMA. To examine ALRX828C anti-inflammatory effect in neuroinflammation, a neurodegenerative model was used to evaluate its potential. I also showed that reducing oxidative stress with a potent antioxidant, Idebenone in nano-emulsion form, can effectively reduce tissue damage during ischemia in organotypic slice culture subjected to oxygen-glucose depravation (OGD). In conclusion, reducing oxidative stress and inflammation after stroke can reduce ischemic damage substantially.

Identiferoai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/17434
Date14 July 2009
CreatorsLiang, Philip
ContributorsCarlen, Peter Louis
Source SetsUniversity of Toronto
Languageen_ca
Detected LanguageEnglish
TypeThesis

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