Parkinsons disease (PD) is a progressive neurodegenerative disorder resulting in part from loss of nigrostriatal dopamine (DA) neurons. Treatments act only to relieve symptoms. It is therefore essential to develop treatments that slow or reverse the neurodegenerative process. Here, I explored exercise as a potential treatment against a 6-hydroxydopamine (6-OHDA) rat model.
6-OHDA causes selective loss of DA neurons, a PD model. Forced limb use after 6-OHDA ameliorates behavioral and striatal DA effects. Further, exercise increases trophic factors, such as GDNF, that have neuroprotective qualities in this model. I explored the effects of forced limb use prior to 6-OHDA on the effects of the toxin and GDNF levels in the striatum. I demonstrated that prior forced limb use attenuated the behavioral deficits and loss of DA typical of 6-OHDA and increased GDNF in the striatum of animals exposed to forced use.
The protective effect of exercise could reflect a decrease in the vulnerability of DA neurons, a regeneration of axons, or sprouting of axon terminals from undamaged neurons. Thus, I investigated the hypothesis that casting induced neuroprotection was due to the preservation of DA cells and terminals. Here, I demonstrated that forced limb use protected from 6-OHDA induced loss of DA neurons and terminals. These findings suggest that exercise exerts its effects by decreasing the vulnerability of DA neurons and terminals to 6-OHDA.
Because casting increased GDNF, I next examined the effects of GDNF on 6-OHDA neurotoxicity during the 8 wk period after 6-OHDA. Using phenotypic markers of the nigrostriatal system, a non-DA cellular marker, and striatal DA content, I demonstrated these markers in the striatum and SN were not protected at 2 wks after 6-OHDA but recovered by 8 wks. No loss of DA cells in the SN or DA content in the striatum was observed in animals pretreated with GDNF. These data suggest that GDNF prevents 6-OHDA-induced DA cell death, but that weeks are required before these cells begin to normally express phenotypic markers. In conclusion, exercise may function to enhance the brains ability to produce trophic factors, which may slow or halt the degenerative process in PD.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-11302006-134350 |
| Date | 11 December 2006 |
| Creators | Cohen, Annie D |
| Contributors | Donald B. DeFranco, Michael J. Zigmond, Teresa G. Hastings, J. Patrick Card, Amanda D. Smith, Brian M. Davis |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-11302006-134350/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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