Orientadora: Profa. Dra. Elizabeth Teodorov / Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Neurociência e Cognição, 2016. / O sistema nervoso central desenvolve-se, em humanos, a partir da terceira semana gestacional e e muito sensivel as influencias ambientais, como, por exemplo, as infeccoes, resultando em danos em longo prazo e muitas vezes irreversiveis, dentre elas o autismo. Devido a incapacidade de expressao de dor, associada a alta taxa de injurias e automutilacoes sofridas por esses pacientes, o mundo cientifico que passou a questionar a veracidade da existencia de hipoalgesia. Buscando-se uma melhor compreensao da dinamica nociceptiva, tanto em aspectos comportamentais quanto moleculares, optou-se pela utilizacao de um modelo animal autista por meio da administracao de LPS no 9,5 dias da gestacao de ratas, a qual induz neuroinflamacao, comprometendo o ambiente neurologico da prole irreversivelmente. Assim, as ratas gestantes tratadas com LPS foram avaliadas quanto a performance reprodutiva, comportamento maternal, sem alteracoes, e expressao de receptores opioides tipo ¿Ê quanto ao gene (Oprm1) e conteudo proteico (MOR) na PAG, onde observou-se um aumento
significativo da expressao de Oprm1 e MOR na PAG das mesmas. As proles (machos e femeas) foram avaliadas quanto ao play behavior aos 21 dias de idade, a hiperalgesia termica e mecanica por meio dos testes Hot Plate e Von Frey, respectivamente, aos 21, 45 e 90 dias de idade e, quanto a expressao de Oprm1 e MOR na PAG aos 90 dias de idade. Em ambas as proles do grupo LPS, ocorreu diminuicao do play behavior, aumento na latencia da resposta de retirada da pata e da intensidade da forca nos testes nociceptivos, e aumento de expressao molecular de Oprm1 e MOR. Conclui-se que a administracao de LPS no 9,5 dias
da gestacao de ratas promove prejuizo no desenvolvimento cerebral das proles, que pode ser evidenciada por alteracoes comportamentais e ativacao de plasticidade no sistema opioidergico. / The central nervous system develops in humans from the third week of gestation and is very sensitive to both genetic and environmental influences such as, for example, the infections, resulting in long term or irreversible damage, among them the autism. Due to the inability of pain expression, associated with increased rates of dammages and self mutilation suffered by these patients, the scientific world began to question the veracity of the existence of hypoalgesia. Seeking for a better understand over the nociceptive dynamics, in both behavioral and molecular aspects, it was decided by the use of an animal model for autism through the administration of LPS on gestational day 9.5 of rats, which induces neuroinflammation, compromising irreversibly the neurological environment of the offspring. Thus, the pregnant rats treated with LPS were evaluated about the reproductive performance, maternal behavior, with no changes, and expression of opioid receptors type ì for gene (Oprm1) and protein product (MOR) in the PAG, with a significant increase in Oprm1 and
MOR expression in their PAG. The offspring derived from these mothers were evaluated for the play behavior at 21 days old, thermal and mechanical hyperalgesia through the Hot Plate test and Von Frey test, respectively, at 21, 45 and 90 days old and over the expression of Oprm1 and MOR in PAG at 90 days old, and both males and females of LPS group showed a decrease of play behavior, increased response latency and intensity of strength in nociceptive
tests, and increased molecular expression for Oprm1 and MOR in PAG. It can be concluded that the LPS administration at 9.5 days of rat gestation promotes cerebral injury of the offspring, which can be evidenced by behavior changes and plasticity enabling the opioid system.
| Identifer | oai:union.ndltd.org:IBICT/oai:BDTD:101141 |
| Date | January 2016 |
| Creators | Carricondo, Érica |
| Contributors | Teodorov, Elizabeth, Bernardi, Maria Martha, Coelho, Cideli de Paula |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf, 101 f. : il. |
| Source | reponame:Repositório Institucional da UFABC, instname:Universidade Federal do ABC, instacron:UFABC |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | http://biblioteca.ufabc.edu.br/index.php?codigo_sophia=101141&midiaext=72381, Cover: http://biblioteca.ufabc.edu.brphp/capa.php?obra=101141 |
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