M.S. / Molecular Biology / Neurospora crassa arg-2 encodes the small subunit of Arg-specific carbamoyl phosphate synthetase and is negatively regulated by arginine. This regulation is mediated by a 24-codon upstream open reading frame (uORF). The sequence of this uORF is critical for Arg-specific regulation. Six mutated templates were used to examine which residues of the uORF are important for this regulation. Mutations were created using megaprimer PCR and a luciferase gene was used as a reporter in the in vitro translation studies. Mutations of Asp 12, Asp 16, and Ser 10 eliminate Arg-specific regulation. Leaky scanning is thought to be involved, and a hypothetical ribosome stalling model that mediates Arg-specific attenuation of translation is proposed.
Identifer | oai:union.ndltd.org:OREGON/oai:content.ohsu.edu:etd/188 |
Date | 06 1900 |
Creators | Carroll, Julie Marie |
Publisher | Oregon Health & Science University |
Source Sets | Oregon Health and Science Univ. Library |
Language | English |
Detected Language | English |
Type | Text |
Format | Needs Adobe Acrobat Reader to view., pdf, 987.203 KB |
Rights | http://www.ohsu.edu/library/etd_rights.shtml |
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