The earthworm has long been of interest to biologists, most notably Charles Darwin, who was the first to reveal their true role as eco-engineers of the soil. However, to fully understand an animal one needs to combine observational data with the fundamental building blocks of life, DNA. For many years, sequencing a genome was an incredibly costly and time-consuming process. Recent advances in sequencing technology have led to high quality, high throughput data being available at low cost. Although this provides large amounts of sequence data, the bioinformatics knowledge required to assemble and annotate these new data are still in their infancy. This bottleneck is slowly opening up, and with it come the first glimpses into the new and exciting biology of many new species. This thesis provides the first high quality draft genome assembly and annotation of an earthworm, Lumbricus rubellus. The assembly process and resulting data highlight the complexity of assembling a eukaryotic genome using short read data. To improve assembly, a novel approach was created utilising transcripts to scaffold the genome (https://github.com/elswob/SCUBAT). The annotation of the assembly provides the draft of the complete proteome, which is also supported by the first RNA-Seq generated transcriptome. These annotations have enabled detailed analysis of the protein coding genes including comparative analysis with two other annelids (a leech and a polychaete worm) and a symbiont (Verminephrobacter). This analysis identified four key areas which appear to be either highly enhanced or unique to L. rubellus. Three of these may be related to the unique environment from which the sequenced worms originated and add to the mounting evidence for the use of earthworms as bioindicators of soil quality. All data is stored in relational databases and available to search and browse via a website at www.earthworms.org. It is hoped that this genome will provide a springboard for many future investigations into the earthworm and continue research into this wonderful animal.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:575937 |
| Date | January 2013 |
| Creators | Elsworth, Benjamin Lloyd |
| Contributors | Blaxter, Mark; Rambaut, Andrew |
| Publisher | University of Edinburgh |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://hdl.handle.net/1842/7596 |
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