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IZOLACE TRANSGENNÍCH ROSTLIN NICOTIANA TABACUM A SILENE VULGARIS / ISOLATION OF TRANSGENIC PLANTS NICOTIANA TABACUM AND SILENE VULGARIS

This project is focused on transformation of Silene vulgaris mediated by Agrobacterium tumefaciens and A. rhizogenes. S. vulgaris is a good model plant to study gynodioecy, an evolutionary step from bisexuality to dioecy. Gynodioecious plants form in some individuals bisexual flowers, while the others possess only female flowers. The aim of this research is do develop a technique to introduce foreign genes into this plant to study its developmental consequences. Using A. rhizogenes we successfuly prepared hairy root cultures, which unfortunately do not form shoot regenerants. We have prepared a protocol to induce plant regenerants from S. vulgaris leaf fragments. The first results do not confirm that A. tumefaciens infected plant regenerants harbor reporter transgenes. We used Nicotiana tabacum as a positive control.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216657
Date January 2010
CreatorsKováčová, Viera
ContributorsDoškař, Jiří, Vyskot,, Boris
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageSlovak
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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