Zou Wei. / Thesis submitted in: December 2001. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 98-119). / Abstracts in English and Chinese. / Declaration --- p.i / Acknowledgements --- p.ii / Publication lists --- p.iii / Abstract --- p.ix / Abbreviations --- p.xiii / List of tables and figures --- p.xiv / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter 1.1. --- Endothelium-dependent relaxation in coronary and pulmonary circulation --- p.1 / Chapter 1.1.1. --- Endothelium-derived relaxing factors --- p.2 / Chapter 1.1.1.1. --- Nitric Oxide --- p.3 / Chapter 1.1.1.2. --- PGI2 --- p.5 / Chapter 1.1.1.3. --- EDHF --- p.6 / Chapter 1.1.2. --- EDHF in coronary and pulmonary circulation --- p.8 / Chapter 1.1.2.1. --- EDHF in coronary circulation --- p.8 / Chapter 1.1.2.2. --- EDHF in pulmonary circulation --- p.9 / Chapter 1.2. --- Effect of hyperkalemia on EDHF-mediated relaxation --- p.10 / Chapter 1.3. --- Organ Preservation Solutions --- p.13 / Chapter 1.3.1. --- Euro-Collins solution --- p.14 / Chapter 1.3.2. --- University of Wisconsin solution --- p.15 / Chapter Chapter 2: --- Objectives and research approaches --- p.16 / Chapter 2.1. --- Objectives --- p.16 / Chapter 2.1.1. --- "Endothelium-dependent relaxation resistant to INDO, L-NNA, and HbO in porcine and pulmonary coronary micro-arteries" --- p.16 / Chapter 2.1.2. --- "EET11,12 and EDHF-mediated function in porcine coronary micro-arteries" --- p.17 / Chapter 2.1.3. --- "Comparison of EC or UW solution on endothelium-dependent relaxation resistant to INDO, l-NNA, and HbO in porcine pulmonary arteries" --- p.17 / Chapter 2.2. --- Research approaches --- p.18 / Chapter 2.2.1. --- "Endothelium-dependence of the relaxation by BK or EET11,12" --- p.18 / Chapter 2.2.2. --- Effect of hypothermic storage with EC and UW solution on EDHF-related relaxation --- p.18 / Chapter 2.2.3. --- Time-dependent alteration of endothelium-dependent relaxation in pulmonary micro-arteries by EC and UW solution --- p.19 / Chapter 2.2.4. --- Effect of HbO in endothelium-dependent relaxation --- p.19 / Chapter Chapter 3: --- Material and Methods --- p.21 / Chapter 3.1. --- General Methods --- p.21 / Chapter 3.1.1. --- Porcine heart and lung collection and transportion / Chapter 3.1.2. --- Myograph --- p.21 / Chapter 3.1.3. --- Myosight --- p.24 / Chapter 3.1.4. --- Anatomizing blood vessel --- p.24 / Chapter 3.1.5. --- Mounting --- p.24 / Chapter 3.1.6 --- Normalization --- p.26 / Chapter 3.1.6.1. --- Normalization of coronary micro-artery --- p.27 / Chapter 3.1.6.2. --- Normalization of pulmonary micro-artery --- p.28 / Chapter 3.1.7. --- Precontraction --- p.30 / Chapter 3.1.8. --- Endothelium-dependent relaxation --- p.31 / Chapter 3.2. --- Coronary artery studies --- p.32 / Chapter 3.2.1. --- Porcine heart harvest and anatomy --- p.32 / Chapter 3.2.2. --- Characteristic of histology of porcine coronary micro-artery --- p.32 / Chapter 3.3. --- Pulmonary artery studies --- p.35 / Chapter 3.3.1. --- Porcine lung harvest and anatomy --- p.35 / Chapter 3.3.2. --- Characteristic of histology of porcine pulmonary micro- artery --- p.36 / Chapter 3.4. --- Drugs --- p.41 / Chapter 3.4.1. --- Drugs --- p.41 / Chapter 3.4.2. --- Preparation of oxyhemoglobin solution --- p.41 / Chapter 3.5. --- Statistical Analysis --- p.42 / Chapter 3.5.1. --- Calculation of EC50 --- p.42 / Chapter 3.5.2. --- Statistical analysis --- p.42 / Chapter Chapter 4: --- "Epoxyeicosatrienoic Acids (EET11,12) May Partially Restore EDHF-Mediated Function in Coronary Micro-Arteries" --- p.43 / Chapter 4.1. --- Abstract --- p.43 / Chapter 4.2. --- Introduction --- p.44 / Chapter 4.3. --- Experimental Protocol --- p.45 / Chapter 4.3.1. --- Precontraction --- p.45 / Chapter 4.3.2. --- "EDHF-mediated (INDO, L-NNA, and HbO-resistant) relaxation" --- p.45 / Chapter 4.3.3. --- "EET11,12-mediated relaxation after exposure to hyperkalemia" --- p.46 / Chapter 4.3.4. --- "The effect of incubation with EET11,12 on the BK-induced, EDHF-mediated relaxation" --- p.46 / Chapter 4.4. --- Results --- p.47 / Chapter 4.4.1. --- Resting force --- p.47 / Chapter 4.4.2. --- HbO and U46619-induced contraction force --- p.48 / Chapter 4.4.3. --- "EET11,12-induced relaxation in coronary micro-arteries after exposure to hyperkalemia" --- p.49 / Chapter 4.4.4. --- "The EDHF-mediated relaxation to BK resistant to INDO, l- NNA,and HbO" --- p.51 / Chapter 4.4.4.1. --- Incubated in either hyperkalemic solution (K+ 20mmol/L) or Krebs' solution (control) --- p.51 / Chapter 4.4.4.2. --- "Incubated in either hyperkalemic solution (K+ 20mmol/L) plus EET11,12 or Krebs' solution (control)" --- p.53 / Chapter 4.5. --- Discussion --- p.57 / Chapter 4.5.1. --- EDHF plays an important role in the coronary micro-arteries --- p.57 / Chapter 4.5.2. --- "EDHF-mediated (INDO, l-NNA, and HbO-resistant) relaxation in the coronary micro-arteries" --- p.58 / Chapter 4.5.3. --- "EET11,12 may partially mimic the EDHF-mediated relaxation in the porcine coronary micro-artery" --- p.59 / Chapter 4.5.4. --- "Effect of EET11,12 added in hyperkalemia may partially restore the EDHF-mediated relaxation in the porcine coronary micro-arteries" --- p.59 / Chapter Chapter 5: --- Impaired EDHF-Mediated Relaxationin Porcine Pulmonary Micro-arteries by Cold Store with Euro-Collin's and University of Wisconsin Solution --- p.61 / Chapter 5.1. --- Abstract --- p.61 / Chapter 5.2. --- Introduction --- p.62 / Chapter 5.3. --- Experimental Protocol --- p.64 / Chapter 5.3.1. --- Precontraction --- p.64 / Chapter 5.3.2. --- "Role of EDHF-mediated (INDO, L-NNA and HbO-resistant) relaxation in porcine pulmonary micro-arteries by BK orA23187" --- p.64 / Chapter 5.3.3. --- Effect of hyperkalemia or preservation solutions (EC or UW) on the EDHF-mediated relaxation by BK or A23187 --- p.65 / Chapter 5.3.3.1. --- The effect of hyperkalemia --- p.65 / Chapter 5.3.3.2. --- Effect of EC solution on the EDHF-mediated relaxation --- p.65 / Chapter 5.3.3.3. --- Effect of UW solution on the EDHF-mediated relaxation --- p.66 / Chapter 5.3.3.4. --- The effect of UW and EC solutions on the contractility of the pulmonary micro-artery --- p.66 / Chapter 5.4. --- Results --- p.66 / Chapter 5.4.1. --- Resting force --- p.66 / Chapter 5.4.2. --- U46619-induced contraction force --- p.67 / Chapter 5.4.3. --- Role of EDHF-mediated relaxation induced by BK or A23187 --- p.67 / Chapter 5.4.4. --- The effect of hyperkalemia --- p.71 / Chapter 5.4.5. --- Effect of EC solution on the EDHF-mediated relaxation --- p.72 / Chapter 5.4.6. --- Effect of UW solution on the EDHF-mediated relaxation --- p.73 / Chapter 5.4.7. --- The effect of UW and EC solution on the contractility of the pulmonary micro-artery --- p.73 / Chapter 5.5. --- Discussion --- p.77 / Chapter 5.5.1. --- EDHF-mediated endothelial function exists in the pulmonary micro-circulation --- p.77 / Chapter 5.5.2. --- Hyperkalemia exposure reduces EDHF-related relaxation and possible mechanism --- p.78 / Chapter 5.5.3. --- The effect of EC and UW solutions on the EDHF-media relaxation in the pulmonary micro-arteries --- p.79 / Chapter Chapter 6: --- General Discussion --- p.82 / Chapter 6.1. --- Endothelium-dependent vasodilators: BK and A23187 --- p.82 / Chapter 6.2. --- EDHF in porcine coronary and pulmonary micro-arteries --- p.84 / Chapter 6.2.1. --- EDHF in porcine coronary micro-arteries --- p.84 / Chapter 6.2.2. --- EDHF in porcine pulmonary micro-arteries --- p.87 / Chapter 6.2.3. --- Vascular stretch and release of endothelium-derived vasodilators --- p.87 / Chapter 6.2.4. --- "EET11,12" --- p.88 / Chapter 6.3. --- "Endothelium-dependent relaxation resistant to INDO, L- NNA, and HbO in porcine coronary and pulmonary microcirculation" --- p.89 / Chapter 6.4. --- "Alteration of endothelium-dependent relaxation resistant to INDO, l-NNA, and HbO after exposure to hyperkalemia" --- p.90 / Chapter 6.5. --- "Alteration of endothelium-dependent contraction resistant to INDO, L-NNA, and HbO after exposure to EC or UW solutions" --- p.91 / Chapter 6.6. --- Clinical implications --- p.92 / Chapter 6.7. --- Limitations --- p.93 / Chapter 6.7.1. --- Common limitations --- p.93 / Chapter 6.7.2. --- Limitation of in vitro study --- p.93 / Chapter 6.8. --- Future work --- p.94 / Chapter Chapter 7: --- Conclusion --- p.96 / References --- p.98 / Appendies / "Wei Zou, Qin Yang, Anthony PC Yim, & Guo-Wei He Epoxyeicosatrienoic acids (EET11,12) may partially restore endothelium- derived hyperpolarizing factor-mediated function in coronary micro- arteries. Annals of Thoracic Surgery. 2001; 72(12): 1970~1976."
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323983 |
| Date | January 2002 |
| Contributors | Zou, Wei., Chinese University of Hong Kong Graduate School. Division of Surgery. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xx, 119, [7] leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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