Two aspects of the biological activity of N-nitrosamines were studied. First, the effect of ascorbate on the mutagenicity of N-nitrosopiperidines was studied in the Ames Salmanella/ mammalian microsome mutagenicity test. The addition of ascorbate significantly enhanced the mutagenicity of these compounds. This enhancement was selective for N-nitrosamines suggesting a possible role of ascorbate in N-nitrosamine induced carcinogenicity. Second, the technique of velocity sedimentation in alkaline sucrose density gradients was applied to the detection of N-nitrosamine induced DNA damage in Balb/c 3T3 cells. This technique detected N-nitrosamine induced DNA damage when the cells were made permeable before treatment. This technique compares favorably with other test systems used to evaluate N-nitrosamines and should be useful in further studies of N-nitrosamines.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc504066 |
| Date | 08 1900 |
| Creators | Barton, Rodney A. (Rodney Alan) |
| Contributors | Jacobson, Myron, Lyle, Bert E., Jr. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 108 leaves: ill., Text |
| Rights | Public, Barton, Rodney A. (Rodney Alan), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
Page generated in 0.0023 seconds