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Two-photon Microscopy and Polarimetry for Assessment of Myocardial Tissue Organization

Optical methods can provide useful tissue characterization tools. For this project, two-photon microscopy and polarized light examinations (polarimetry) were used to assess the organizational state of myocardium in healthy, infarcted, and stem-cell regenerated states. Two-photon microscopy visualizes collagen through second-harmonic generation and myocytes through two-photon excitation autofluorescence, providing information on the composition and structure/organization of the tissue. Polarimetry measurements yield a value of linear retardance that can serve as an indicator of tissue anisotropy, and with a dual-projection method, information about the anisotropy axis orientation can also be extracted. Two-photon microscopy results reveal that stem-cell treated tissue retains more myocytes and structure than infarcted myocardium, while polarimetry findings suggest that the injury caused by temporary ligation of a coronary artery is less severe
and more diffuse that than caused by a permanent ligation. Both these methods show
potential for tissue characterization.

Identiferoai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/25410
Date14 December 2010
CreatorsArchambault-Wallenburg, Marika
ContributorsVitkin, Alex
Source SetsUniversity of Toronto
Languageen_ca
Detected LanguageEnglish
TypeThesis

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