Stem cell therapy is a promising approach for tissue regeneration but there exists a
problem of low engraftment rate to the injury site. Our laboratory has shown that
hMSCs that were capable to penetrate through collagen barrier have higher
migration capacity and engraftment efficiency than those remained inside the
collagen matrix and those in traditional 2D culture. These cells capable of
penetrating through collagen barrier might be hopeful candidate for improving
engraftment efficacy. Major processes of engraftment, such as transmigration
through basement membrane and invasion to the site of injury, involve
cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP)
and integrins are the key players in these processes, MMP and integrin profiles of
the hMSCs were studied
In this study, we demonstrated that hMSCs that were capable of penetrating
through the collagen barrier have distinctive MMP profile to traditional 2D culture.
These cells secrete significantly higher amount of MMP-1 than 2D culture, but the
amount of MMP-2 secreted is comparable to traditional 2D culture. On the other
hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups.
Moreover, we have investigated the subcellular localization of MMPs and
integrins. The cells were seeded on dishes with or without ECM coatings. It was
demonstrated that hMSCs capable of penetrating through collagen barrier exhibit
higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish.
Moreover, these cells exhibit a prominent feature of perinuclear localization of
MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is
comparable to that in 2D culture.
We have also investigated the stem properties of hMSCs penetrated through
collagen barrier. These properties include proliferation capacity, self-renewal
capacity and differentiation potential towards chondrogenic, osteogenic and
adipogenic lineages. It has been demonstrated that these properties are not
compromised for superior migratory activities. / published_or_final_version / Mechanical Engineering / Master / Master of Philosophy
| Identifer | oai:union.ndltd.org:HKU/oai:hub.hku.hk:10722/161540 |
| Date | January 2011 |
| Creators | Wong, Yin-kwai., 王現葵. |
| Contributors | Chan, BP |
| Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
| Source Sets | Hong Kong University Theses |
| Language | English |
| Detected Language | English |
| Type | PG_Thesis |
| Source | http://hub.hku.hk/bib/B47869720 |
| Rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works., Creative Commons: Attribution 3.0 Hong Kong License |
| Relation | HKU Theses Online (HKUTO) |
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