Nonribosomal peptides (NRP) are highlighted in pharmacological studies as novel NRPs are often promising substances for new drug development. To effectively discover novel NRPs from microbial fermentations, a crucial step is to identify known NRPs in an early stage and exclude them from further investigation. This so-called dereplication step ensures the scarce resource is only spent on the novel NRPs in the following up experiments. Tandem mass spectrometry has been routinely used for NRP dereplication. However, few bioinformatics tools have been developed to computationally identify NRP compounds from mass spectra, while manual identification is currently the roadblock hindering the throughput of novel NRP discovery.
In this thesis, we review the nature of nonribosomal peptides and investigate the challenges in computationally solving the identification problem. After that, iSNAP software is proposed as an automated and high throughput solution for tandem mass spectrometry based NRP identification. The algorithm has been evolved from the traditional database search approach for identifying sequential peptides, to one that is competent at handling complicated NRP structures. It is designed to be capable of identifying mixtures of NRP compounds from LC-MS/MS of complex extract, and also finding structural analogs which differ from an identified known NRP compound with one monomer. Combined with an
in-house NRP structural database of 1107 compounds, iSNAP is tested to be an effective tool for mass spectrometry based NRP identification.
The software is available as a web service at http://monod.uwaterloo.ca/isnap for the research community.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OWTU.10012/6641 |
| Date | 19 April 2012 |
| Creators | Yang, Lian |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
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