Catfish skin (CS) contains considerable amount of protein that may be hydrolyzed enzymatically to generate hydrolysates (CSH) which may have antioxidant and antimicrobial properties. The generation of natural compounds with these properties is of special interest to the food industry. The objective of this study was to develop and characterize CSH including antioxidant and antimicrobial properties. CSH were produced from different enzymatic reaction periods using alkaline protease, and their characterization, antioxidant, antimicrobial, and some functional properties were evaluated. A selected CSH was analyzed for peptide composition and partial amino acid sequences. The color analysis showed that darker, more yellow, and redder CSH were generated throughout the time of hydrolysis. CSH at 4, 5, and 6 h of hydrolysis showed considerable antioxidant activity and antimicrobial properties in-vitro, high emulsion stability, and low oil holding capacity influenced by the time of hydrolysis. Low-molecular weight peptides with positive charges were suggested as responsible for the antimicrobial activity. CSH were developed and characterized. Further research is suggested, especially on identification, isolation, and synthesis of specific peptides from CSH with both antioxidant and antimicrobial activity.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-11142016-091144 |
| Date | 08 December 2016 |
| Creators | Galindo Perez, Cesar Augusto |
| Contributors | Sathivel, Subramaniam, King, Joan, Bechtel, Peter, Bankston, David |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-11142016-091144/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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