Because of their health benefits, probiotics are a significant part of the functional food industry. Spray drying is reported as the most common method used in the food industry to encapsulate probiotics. The objectives of this study were to investigate the effects of protective agents on the viability of lactobacilli after spray drying and during storage at different conditions and to evaluate spray drying conditions to produce these probiotic powders. Lactobacillus plantarum NRRL B-4496 (LP) and Lactobacillus acidophilus NRRL B-4495 (LA) were separately grown (~109 CFU/ml) and suspended in a 200 g/L solution of high maize starch (HM); maltodextrin (MD); or gum arabic (GA). The solutions were separately spray dried at 140 °C to obtain LP and LA-powders: LPHM, LPMD, LPGA, LAHM, LAMD, and LAGA. The powders were separately placed in aluminum bags and separately packed under 97% and 10% vacuum. The powders were stored at refrigerated (4 °C) or at room (23 °C) temperature for 60 days. Physicochemical properties, energy and mass balances, and cell viability during storage were determined. Triplicate experiments were conducted and data were statistically analyzed (α=0.05). The actual production rate of powders ranged from 0.091 to 0.105 (kg dry solids/h). The energy used during spray drying was not significantly different for any of the powders. After 60 days, LPHM powders packed under 10% and 97% vacuum and stored at 4 °C had significantly higher cell viability than the other powder samples. The study demonstrated significantly improved on the viability of LP at 10% vacuum stored at refrigerated temperature for the HM treatment compared to those treated with MD and GA. The data obtained showed that high maize starch can be used as a protective agent to maintain the viability of L. plantarum powder at recommended levels for up to 60 days of storage.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-07102017-144306 |
| Date | 25 July 2017 |
| Creators | Reyes Ortega, Vondel Vandeker |
| Contributors | Sathivel, Subramaniam, Keenan, Michael J., Bankston, J. David |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-07102017-144306/ |
| Rights | withheld, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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