ABSTRACT Aim: To understand endodontic infections and their prognosis knowledge of which bacteria are present, viable, and part of perpetuating the late stages of the infection. The aim of this study was to investigate three different methods for mapping the composition of the microbiota in infected root canals. Methods: (i)Culturing on agar discs; (ii)real-time polymerase chain reaction (qPCR) processing; and (iii)qPCR processing of samples pre-treated with propidium monoazide (PMA). Samples were collected from 32 teeth and stored in buffer solution. Culturing was conducted on Fastidious Anaerobe Agar plates using 200 µL sample solution. Another 400 µL was treated with PMA dye and photoactivation prior to qPCR processing, while the remaining 400 µL was processed by qPCR without pretreatment. The sampled diagnoses were asymptomatic apical periodontitis, asymptomatic apical periodontitis with sinus tract, and symptomatic apical periodontitis. Results: The study found no significant difference in amount of bacteria between the three diagnoses, but found that different methods yielded significantly different results when applied to each diagnosis. qPCR detected the highest absolute numbers of bacteria, and qPCR with PMA the lowest. Conclusions: Results indicate that different methods yield different results, but that the diagnoses do not differ much in terms of bacterial load. To confidently conclude whether or not there is a connection between diagnosis and amount of bacteria, a larger sample size is needed. The results in this study are suspected to be skewed by compromised data, and no certain conclusions can be drawn.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:umu-179535 |
| Date | January 2020 |
| Creators | Påhlson, Emma, Bergdahl, Alexander |
| Publisher | Umeå universitet, Tandläkarutbildning, Umeå universitet, Tandläkarutbildning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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