Mammalian Cut proteins appear to function as transcriptional repressors that play a role in determining cell type specificity. Recent evidence suggests that cut genes are tumour suppressor genes. This hypothesis was further examined by using functional assays to evaluate the abilities of human Cut to: (a) suppress oncogene-mediated cell transformation, and (b) interact with the SV40 Large T (LT) antigen. The results indicate that: (a) there was a decrease in the number of transformed colonies and foci when rat embryo fibroblasts (REFs) were transfected with ras/c-myc or ras/E1A and cut; (b) the homeodomain region of Cut interacted with SV40 LT in vitro; and (c) Cut protein levels were elevated and Cut DNA binding was increased in cells transfected or transformed with SV40LT. These observations suggest that Cut proteins may regulate cell proliferation and that the Cut homeodomain may mediate protein interactions.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27536 |
| Date | January 1997 |
| Creators | Kelman, Marni J. |
| Contributors | Nepveu, Alain (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001601809, proquestno: MQ37135, Theses scanned by UMI/ProQuest. |
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