Due to its significant morbidity, alternatives to autogenous bone grafting are required. Recent research has focused on application of growth factors, including bone morphogenetic proteins (BMPs). Two recombinant human BMP (rhBMP) containing bioimplants, Infuse® (rhBMP-2) and OP-1® (rhBMP-7), are approved for human application. Objective: to provide a direct comparison of the two approved rhBMP containing bioimplants in their clinically available forms. Methods: Activity of rhBMP-2 and -7 were tested using the C2C12 cell based assay comparing alkaline phosphatase (ALP) activity. Activity of Infuse® or OP-1® bioimplants containing 52.5µg of rhBMP-2 or rhBMP-7 were compared using a mouse muscle pouch assay and analyzed with micro CT (mCT) and histology. Results: in vitro: rhBMP-2 induced greater ALP production than rhBMP-7 at different time points. In vivo: OP-1® induced greater bone volume than Infuse® of equivalent quality based on mCT. Conclusions: In the clinically available form, OP-1® induced greater bone volume than Infuse®.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/26533 |
| Date | 30 March 2011 |
| Creators | Barr, Torin Richard |
| Contributors | Clokie, Cameron |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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