Attempts were made to explore the possibility of haploid plant production in oats (Avena sativa L.) through in vitro techniques. For anther culture, among 89 entries, only four cultivars, namely, Cayuse, Manod, Stout and Tarpan, produced calluses. Serial sections of the callusing anthers revealed the pollen origin of these calluses. Genotypic differences in anther response in oats were confirmed. Aborted multicellular structures were observed in the pollen sacs of 12 cultivars/strains. Further improvement of the medium is necessary to ensure the continual development of initiated pollen grains to plantlet formation. Cultivar differences in the frequency of non-stained (NS) grains were observed. Cold pre-treatment did not increase the frequency of NS or abnormal bi-nucleate grains. The possible effect of cold pre-treatment is to delay anther deterioration, which ensures the survival of more grains to switch into embryogenesis. / For ovary and panicle culture, no callus formation was obtained. The potential of the latter approach is discussed.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68551 |
| Date | January 1980 |
| Creators | Chung, Siu-Wah. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Plant Science) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000593951, proquestno: AAINK51910, Theses scanned by UMI/ProQuest. |
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