Estradienolone (ED, 17beta-hydroxy-[1,5]-estradien-3-one) is a novel steroid hormone identified in pregnant women. It is an isomer of estradiol which binds the progesterone receptor and sex hormone binding globulin with high affinity. Since levels of ED remain high throughout pregnancy and decrease in association with term or preterm labour, ED may be involved in the maintenance of pregnancy. The objectives of the present study were to, (i) purify ED from pregnancy samples in quantities sufficient for chemical analysis to confirm its structural features, and (ii) identify its binding proteins and receptors. Our results show that ED binds the novel methyltrienolone (R1881) binding protein of JEG-3 cells with higher affinity than any other natural steroids tested. Furthermore, ED is able to inhibit transcriptional activity of the estrogen-receptor-related receptors alpha and gamma, suggesting that ED may be the natural ligand of these orphan receptors. ED is also able to activate the xenobiotic receptor SXR. Chemical analysis of the fine structural features of purified ED is in progress.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.82297 |
| Date | January 2003 |
| Creators | Negi, Ranuka |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002499635, proquestno: AAIMR12508, Theses scanned by UMI/ProQuest. |
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