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Alterations in gene expression in the oomycete Achlya ambisexualis

Differentiation and gene expression in the oomycete Achlya ambisexualis ( strain E87) were studied during three responses, sexual antheridium formation, asexual sporangium formation and heat shock. Polyacrylamide gel electrophoresis of proteins labelled in vivo and in cell free systems directed by poly(A) mRNA showed that during formation of antheridia only one change was detectable in the proteins synthesized. No detectable changes were observable in the translatable poly(A) RNA population indicating that post-translational events may be required for differentiation. During early sporangium formation several proteins showed changes in relative rates of synthesis. These correlate with similar changes in cell free translation products suggesting a transcriptional level of regulation. Nucleic acid hybridization analyses of mRNA populations indicated that many new sequences appear late and accumulate in the spore. These late transcripts are probably utilized during spore germination. During heat shock several proteins showed rapid increases in rates of synthesis; these seem to be controlled at the level of transcription.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.77135
Date January 1981
CreatorsGwynne, David I. (David Ivor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Biology)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000139593, proquestno: AAINK58032, Theses scanned by UMI/ProQuest.

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