The study of the in vitro circadian oscillator of the cyanobacterium
Synechococcus elongatus has uncovered a complex interplay of its three protein
components. Synchronization of the clock's central oscillatory component, KaiC, has
been thought to be achieved through subunit shuffling at specific intervals during the
clock's period. By utilizing an established fluorescence-based analysis on completely
phosphorylated and dephosphorylated mutants as well as wild-type KaiC, this study has
shown that shuffling rates are largely unaffected by phosphorylation state. These
findings conflict with previous reports and hence revise our understanding of this
oscillator.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/85785 |
| Date | 10 October 2008 |
| Creators | Ihms, Elihu Carl |
| Contributors | LiWang, Andy C. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | electronic, born digital |
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