OBJECTIVES: Bacterial infections in the normally sterile environment of the middle ear cavity
usually trigger host immune response, whereby the innate immune system plays
a dominant role as the host’s first line of defense. In this study we evaluated the
expression levels of Toll-like receptors (TLRs) -2, -4, -5, -9, and nucleotidebinding
oligomerization domain-containing proteins (NODs) -1 and -2, all of which
are related to bacterial infection in pediatric patients with otitis media with
effusion (OME).
METHODS: The study sample consisted of 46 pediatric patients with OME, all of whom had
ventilation tubes inserted. The expression levels of TLR-2, -4, -5, -9, NOD-1 and
-2 mRNA in middle ear effusion were assessed by polymerase chain reaction.
RESULTS: All effusion fluid samples collected from patients with OME showed expression of
TLR-2, -4, -5, -9, NOD-1, and -2 mRNA. However, we found no correlations
among expression levels of pattern recognition receptors (PRRs) in relation to
characteristics of exudates, presence of bacteria, or frequencies of ventilation
tube insertion (p>0.05).
CONCLUSION: Our findings suggest that exudates of OME patients show PRR expressions that
are related to the innate immune response regardless of the characteristics of
effusion fluid, presence of bacteria in exudates, or frequency of ventilation tube
insertion.
| Identifer | oai:union.ndltd.org:bu.edu/oai:open.bu.edu:2144/17154 |
| Date | January 2013 |
| Creators | Lee, So Yoon |
| Source Sets | Boston University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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