The main goal in my project was find out if the rate of zinc crossing the bilayer was either due to the fluidity of vesicles or the level of oxidation of the vesicles.To measure the oxidation a simple procedure called the TBA Test was used to measure each PC tested. The fluidity measurement was a calculation using the temperature the vesicles went from gel to liquid crystalline phase and the experimental temperature.Measuring the rate at which zinc crossed the bilayer was done using spectral changes that occur as zinc binds with APIII, a metal chelator entrapped inside the vesicles. To measure these rates we used k', the rate constant at which zinc is crossing the bilayer at a certain concentration and k, the second order diffusion rate constant which is the slope of k' vs. [Zn].The rates at which zinc was crossing the bilayer for each PC was then compared to the fluidity and oxidation levels for each PC. There was no direct correlation between the rates and fluidity but there was a good linear correlation between the rates and oxidation.So it seemed as if oxidation was the main reason zinc was crossing the bilayer so we wanted to see if our measurements could be obtained by biological cells. The comparison showed that rates obtained by biological cells can only be matched by the vesicle models when there oxidation levels are found to be high.In conclusion we believe that the reason zinc is crossing the bilayer is due to oxidation that occurs to the vesicle and as oxidation increases so do the rates at which zinc crosses the bilayer. / Department of Chemistry
Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/185943 |
Date | January 1997 |
Creators | Telles, Scott Gerard |
Contributors | Ball State University. Dept. of Chemistry., Pattison, Scott E. |
Source Sets | Ball State University |
Detected Language | English |
Format | vii, 62 leaves ; 28 cm. |
Source | Virtual Press |
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