There is increasing evidence that hydrogen peroxide (H2O2) can act as a signalling molecule capable of modulating a variety of biochemical and genetic systems. Using Jurkat T-lymphocytes, this study initially investigated the involvement of H2O2 in the activation of a specific signalling protein extracellular signal-regulated protein kinase (ERK). It was found that as a result of H2O2 treatment, mitochondrial complex activities decreased which led to subsequent increase of mitochondrial reactive oxygen species (ROS) production. The increase of ROS resulted in higher cellular H2O2 as well as increased ERK activation. This study demonstrated that in an oxidative stress setting, H2O2 production from the mitochondria was an essential component in maintaining the activation of a signalling protein. One way in which H2O2 could influence protein function is by the oxidation of susceptible thiol groups of cysteine residues. To further understand the variety of signalling pathways that H2O2 may be involved in, an improved proteomics technique was developed to globally identify proteins with susceptible thiol groups. The
| Identifer | oai:union.ndltd.org:ADTP/178808 |
| Date | January 2008 |
| Creators | Lui, James Kwok Ching |
| Publisher | University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia. Biochemistry and Molecular Biology Discipline Group |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
| Rights | Copyright James Kwok Ching Lui, http://www.itpo.uwa.edu.au/UWA-Computer-And-Software-Use-Regulations.html |
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