<p> The present investigation was concerned with mitochondrial energy transfer reactions and their relationship to mitochondrial structural integrity. Experiments with azide demonstrated a close relationship between oxidative phosphorylation and large amplitude
mitochondrial volume changes. Azide inhibited energy transfer and energy-linked mitochondrial swelling by competing with adenine nucleotide for a site on the terminal phosphorylating enzyme. As a permeant anion azide exerted secondary effects on mitochondrial structure and function.</p> <p> Experiments with mitochondria treated with phlorizin and phloretin emphasized the importance of Mg++ as a controlling factor in maintaining the integrity of mitochondrial energy transfer processes. The results indicated that these compounds interfered directly with oxidative phosphorylation, and that mitochondrial swelling was either a consequence of impaired energy transfer, or a separate phenomenon.</p> / Thesis / Doctor of Philosophy (PhD)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/20584 |
| Date | 09 1900 |
| Creators | Vigers, Gary Alexander |
| Contributors | Ziegler, F. D., Biochemistry |
| Source Sets | McMaster University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
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