The carbonate radical anion CO3•- is a potent reactive oxygen species (ROS) produced in vivo through enzymatic one-electron oxidation of bicarbonate or, mostly, via the reaction of CO2 with peroxynitrite. Due to the vitally essential role of the carbon dioxide/bicarbonate buffer system in regulation of physiological pH, CO3•- is arguably one of the most important ROS in biological systems. So far, the studies of reactions of CO3•- with DNA have been focused on the pathways initiated by oxidation of guanines in DNA. In this study, low-molecular products of attack of CO3•- on the sugar-phosphate backbone in vitro were analyzed by reversed phase HPLC. The selectivity of damage in double-stranded DNA (dsDNA) was found to follow the same pattern C4′ > C1′ > C5′ for both CO3•- and the hydroxyl radical, though the relative contribution of the C1′ damage induced by CO3•- is substantially higher. In single-stranded DNA (ssDNA) oxidation at C1′ by CO3•- prevails over all other sugar damages. An approximately 2000-fold preference for 8-oxoguanine (8oxoG) formation over sugar damage found in our study identifies CO3•- primarily as a one-electron oxidant with fairly low reactivity toward the sugar-phosphate backbone.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-16656 |
| Date | 11 September 2015 |
| Creators | Roginskaya, Marina, Moore, T. J., Ampadu-Boateng, D., Razskazovskiy, Y. |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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