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Effect of oxygen supply during growth on the production of enzymes, cytochromes and acid end-products by Haemophilus parasuis

Haemophilus parasuis was grown in cystine-supplemented tryptone-yeast extract medium (TYE) sparged with CO$ sb2$-containing gas mixtures. The organism was found to metabolize glucose via acetate- and succinate-producing pathways and a complete tricarboxylic acid cycle was absent. As compared with the oxygen-sufficient organism, oxygen-restricted H. parasuis synthesized higher amounts of high and low potential cytochromes c, exhibited higher activities of the enzymes of the succinate-producing pathway and excreted less acetate and more formate and succinate. The low potential cytochromes c were reduced by NADH and oxidized by fumarate and were situated on both sides of the HOQNO inhibition site of the low potential portion of the electron transport chain, but did not seem strictly required for the NADH:fumarate oxidoreductase activity. It is suggested that the low potential cytochromes c might act as an electron buffer permitting the regeneration of NAD under conditions of scarcity of the final electron acceptor.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74613
Date January 1991
CreatorsMartin, Pierre Gaétan
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Microbiology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001221847, proquestno: AAINN67639, Theses scanned by UMI/ProQuest.

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