Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its anti-apoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-κB inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84 mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69 mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx meanwhile decreased the expressions of p-NF-κB/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-κB/p65. We concluded that ROS, NF-κB and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-κB and COX-2.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-18479 |
| Date | 03 August 2009 |
| Creators | Liu, Xiao J., Shi, Shao T., Ye, Jun L., Liu, Le Q., Sun, Mi, Wang, Chun Bo |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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