<p><p>POOLPERM, PARIWAT. Factors Influencing Semen Quality and Fertilityin Boars. (Under the direction of Drs.Glen W. Almond and William L. Flowers)<p> The objectives of this researchwere 1) determine the influence of antibiotics on semen quality, 2) determinethe association among insulin-like growth factor I (IGF-I) in seminal plasma,semen quality, and subsequent fertility, and 3) retrospectively study theassociation among semen parameters and sow fertility. In the firststudy, the effects of gentamicin (GM), amikacin (AM), neomycin sulfate(NM), and penicillin-streptomycin (PS) in semen extender on the percentagesof motile (MOT), morphologically normal sperm (MOR), and sperm with normalacrosome (NAR) were examined. An in vitro penetration assay was conductedusing sperm cells on day 0 and day 5 of storage. GM and NM groupsshowed higher (p<0.05) MOT than other groups after 5 days of storage. No differences in penetration rate were found among treatments; however,the penetration rate decreased (p<0.05) on day 5 of storage.<br> In the second study, ejaculateswere collected and diluted in an extender (Vital?). Gilts (n=113)and sows (n=375) were inseminated with the extended semen in homogenetic-homospermicregimens. Farrowing rate (FR), total pigs born (TB) and born alive(TBA) were recorded. IGF-I was determined in seminal plasma by radioimmunoassay. Concentration of IGF-I from 204 ejaculates was 95.38 ± 3.56 ng/ml (mean± SEM) and total amount of IGF-I/ejaculate was 23.50 ± 1.20 µg. SeminalIGF-I differed (p<0.05) among genetic lines and had no effect (p>0.05)on MOT, MOR and NAR. However, IGF-I was associated (p<0.05) withsemen volume, sperm concentration and total number of sperm/ejaculate. No association between IGF-I level in seminal plasma and fertility indiceswas found.<br> The third study determinedthe associations among insemination parameters with subsequent fertilityof boar semen using data from the second study. MOR was associated(p<0.05) with fertility parameters. TB and TBA were associatedwith age of semen at the first insemination (SAGE) and number of spermper insemination dose (AIDOSE). With stepwise regression analysis,it was evident that FR was associated with semen volume, MOR and SAGE. Meanwhile, TB and TBA were associated with SAGE, AIDOSE and total numberof spermatozoa/ejaculate. In conclusion, the assessment of semencharacteristics may not necessarily delineate fertility between boars.<P>
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-20010831-112730 |
| Date | 31 August 2001 |
| Creators | Poolperm, Pariwat |
| Contributors | Dr.Glen W. Almond, Dr.William L. Flowers, Dr.William L. Miller, Minor-representative committee, Dr.Peter W. Farin, Committee member |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-20010831-112730 |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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