Abstract
The genetic basis of large adaptive differences in timing
of bud set and frost hardiness between natural populations of Scots
pine (Pinus sylvestris L.) was studied with
the aid of RAPD markers and quantitative genetic tools. Steep clinal
variation was found for both traits among Finnish Scots pine populations,
and the differences between populations were found to be largely
genetic. QTL mapping with Bayesian analysis revealed four potential
QTLs for timing of bud set, and seven for frost hardiness. The QTLs
were mostly different between the two traits. The potential QTLs
included loci with large effects, and additionally smaller QTLs.
The largest QTLs for bud set date accounted for about a fourth of
the mean difference between populations. Thus, natural selection
during adaptation has resulted in fixation of genes of large effect.
This result is in conflict with the classical infinitesimal model,
but agrees with the results of Orr (1998), suggesting fixation of
large effects during adaptation.
The applicability of RAPD and SSCP markers in quantitative
genetic studies was also studied. The SSCP technique was found to
be efficient in finding polymorphic markers. SSCP polymorphism in
coding genes may provide candidate genes for QTL mapping studies.
RAPDs were found to be useful for many descriptive analyses, but
specific analyses would require more caution.
| Identifer | oai:union.ndltd.org:oulo.fi/oai:oulu.fi:isbn951-42-5504-6 |
| Date | 21 December 1999 |
| Creators | Hurme, P. (Päivi) |
| Publisher | University of Oulu |
| Source Sets | University of Oulu |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis, info:eu-repo/semantics/publishedVersion |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess, © University of Oulu, 1999 |
| Relation | info:eu-repo/semantics/altIdentifier/pissn/0355-3191, info:eu-repo/semantics/altIdentifier/eissn/1796-220X |
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