Sun lower (Helianthus annuus) is a commercially important crop plant.
Susceptible plants in ected with lea rust (Puccinia helianthi) leads to
great yield losses. The aim o this study was to identi y putative protein
kinase genes rom a resistant sun lower cultivar that are involved in the
early resistance response when the plant is in ected with lea rust.
Several di erentially expressed cDNA ragments were isolated rom
in ected resistant sun lower. One o these identi ied clones (D15)
showed an 8.5 old induction o gene expression 3 h.p.i. in in ected
resistant sun lower, while the expression in the susceptible in ected
cultivar was repressed. A second 31 old activation was ound 15 h.p.i.
This second induction most probably orms part o induced SAR in the
unin ected plant tissue. This cDNA clone showed homology to several
protein kinase genes. In addition, the gene is present as a single copy
within the genomes o susceptible and resistant sun lowers. A
polymorphism in size was however evident between the genes present
in the two cultivars.
Three other cDNA clones were also selected on the basis o their
expression pro iles. These cDNAs showed homology to Apyrases, a
Rac-protein and Xyloglucan endotransglycosylases respectively. The
involvement o all our cDNAs in the activation o plant de ense
however still needs to be con irmed.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-09072005-085410 |
| Date | 07 September 2005 |
| Creators | Bezuidenhout, Mariette |
| Contributors | Dr B visser, Prof AJ van der Westhuizen |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-09072005-085410/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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