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Previous issue date: 2013-04-05 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Recently, marine organisms have attracted attention because of the
complexity and potent biological activity from your secondary metabolites. Our
planet has 80% it surface covered by oceans and seas, therefore, housing a
wide number of different forms of life, among them, the sponges. These sessile
and filtrating animals, according to numerous researches, come showing like
true chemistry factories. The substances from these animals, sometimes show
as news targets to therapeutics agents, and some countries has already use
them for treatment of some diseases. Further of the secondary metabolites, the
polysaccharides of marine origin also have been target of studies, because the
presence of the sulfates groups in its molecules. Polysaccharides with differents
biological activities have been related in a large number of researches. Actually,
many studies show the sponges as source of promising medicine. These
studies inspire new researches, because the few number of sponges species
studied until now. Because of that, the present work shows the chemistry
prospection of the sponge Callyspongia vaginalis. Chromatographic methods in
silica gel allowed the isolations of two secondary metabolites: the known β-
sitosterol and a ceramide, no reported in the genus Callyspongia, previously.
The analysis of the their lipid extracts show different kinds of fatty acids with a
variety of chain length (saponifiable fraction), and others metabolites like
Lupenone and stigmasterol, also unprecedented in the genus. The
Polysaccharide characterization and the elucidation of the secondary
metabolites acquired through of chromatography analysis (CC, molecular
exclusion) and spectrometric (NMR 1H and 13C, mass, IR), respectively and
comparison with literature data / A diversidade estrutural de subst?ncias de origem marinha, associadas
as suas atividades biol?gicas, t?m atra?do aten??o de pesquisadores do mundo
inteiro. Dentre os organismos estudados atualmente dois grupos de
invertebrados marinhos, as asc?dias e as esponjas, merecem destaque. Estes
animais s?sseis e filtradores, segundo in?meras pesquisas, v?m se mostrando
verdadeiras f?bricas qu?micas , produzindo subst?ncias que por vezes
apresentam-se como aspirantes a agentes terap?uticos, com efic?cia cl?nica
comprovada. Al?m dos metab?litos secund?rios, os polissacar?deos de origem
marinha tamb?m tem sido alvo de estudos, sendo relatados diversos
compostos sulfatados e com diferentes atividades biol?gicas associadas a
estes. Tendo em vista a pequena quantidade de esp?cies de esponjas
estudadas at? o momento, comparado ao potencial qu?mico destes
organismos, o presente trabalho apresenta a prospec??o qu?mica da esponja
Callyspongia vaginalis. T?cnicas cl?ssicas de cromatografia em gel de s?lica
permitiram o isolamento de dois metab?litos secund?rios: o β-sitosterol e uma
ceramida, ambos in?ditos no g?nero Callyspongia. A an?lise das fra??es
lip?dicas mostraram diferentes tipos de ?cidos graxos com variados tamanhos
de cadeia (fra??o saponific?vel), e de outros metab?litos como lupenona e
estigmasterol, tamb?m in?ditos no g?nero em quest?o. O polissacar?deo obtido
atrav?s do material animal tamb?m foi isolado e, como amplamente relatado na
literatura apresentou-se sulfatado, caracter?stica diferencial dos polissacar?deos
de origem marinha. A caracteriza??o do polissacar?deos e a elucida??o
estrutural dos metab?litos secund?rios foram realizadas atrav?s da an?lise dos
dados de espectrometria de massa, Infravermelho e Resson?ncia Magn?tica
Nuclear de Hidrog?nio-1 e Carbono-13, atrav?s de sequ?ncias de pulsos uni e
bidimensionais e compara??o com dados da literatura
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17733 |
| Date | 05 April 2013 |
| Creators | Ara?jo, Rusceli Diego de |
| Contributors | CPF:61438669372, http://lattes.cnpq.br/4163879756935913, Pontes, Ana Cristina Facundo de Brito, CPF:42396441353, http://lattes.cnpq.br/0435570420479356, Barreto, Jacqueline do Carmo, CPF:52496830378, http://lattes.cnpq.br/5193184756509698, Langassner, Silvana Maria Zucolotto, CPF:81995725072, http://lattes.cnpq.br/7390416147619446, Ara?jo, Renata Mendon?a |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Qu?mica, UFRN, BR, F?sico-Qu?mica; Qu?mica |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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